Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,691	C→T	A343T (GCC→ACC)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,691	0	C	T	100.0%	62.7 / NA	19	A343T (GCC→ACC)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

AGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGAAATC  >  NC_000913/4353622‑4353763                                                                      |                                                                         aGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGttt                                                               <  1:799315/81‑1 (MQ=255)      ttCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGa                                                          >  2:799337/1‑81 (MQ=255)        ctctACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGAc                                                         >  2:799342/1‑80 (MQ=255)        ctctACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGAc                                                         >  2:799339/1‑80 (MQ=255)             ccGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGatat                                                    <  1:799316/80‑1 (MQ=255)              cGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATAttt                                                  <  2:799317/81‑1 (MQ=255)              cGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATAttt                                                  <  2:799318/81‑1 (MQ=255)              cGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATAttt                                                  >  1:799348/1‑81 (MQ=255)                           gATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTcg                                     <  1:799319/81‑1 (MQ=255)                               cAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCa                                 >  1:799351/1‑81 (MQ=255)                               cAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCACGCa                                 >  2:799347/1‑81 (MQ=255)                                     aTGCTTTAGCAGCATCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGa                           <  1:799320/81‑1 (MQ=255)                                                  aTCAAAATTATCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACgg              >  1:799356/1‑81 (MQ=255)                                                           aTCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGa      <  1:799323/80‑1 (MQ=255)                                                           aTCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGa      <  2:799321/80‑1 (MQ=255)                                                           aTCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGa      <  1:799322/80‑1 (MQ=255)                                                            tCCAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGaaa    >  2:799346/1‑81 (MQ=255)                                                             ccAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGAAAt   >  2:799345/1‑81 (MQ=255)                                                              cAGGTCGGTCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGAAATc  <  2:799324/81‑1 (MQ=255)                                                                      |                                                                         AGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACGGTTTGCCGAAATC  >  NC_000913/4353622‑4353763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑