Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,150	A→G	intergenic (‑216/+401)	cadC ← / ← pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,150	0	A	G	100.0%	38.7 / NA	12	intergenic (‑216/+401)	cadC/pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

ATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGC  >  NC_000913/4362072‑4362226                                                                               |                                                                             aTAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAgaagaa                                                                            <  2:800755/81‑1 (MQ=255)  tAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAAt                                                                           <  1:800756/81‑1 (MQ=255)          cGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGa                                                                    >  1:800773/1‑80 (MQ=255)           gAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGaa                                                                   >  1:800757/1‑80 (MQ=255)           gAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGaa                                                                   >  1:800777/1‑80 (MQ=255)             aaGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAaat                                                                <  2:800758/81‑1 (MQ=255)                     tACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCAt                                                         <  1:800759/80‑1 (MQ=255)                                 gAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCg                                            <  1:800760/81‑1 (MQ=255)                                    gTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAg                                         >  2:800774/1‑81 (MQ=255)                                     tAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGc                                        <  2:800761/81‑1 (MQ=255)                                            ccTTACCATCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTAc                                  <  2:800762/80‑1 (MQ=255)                                                    tCAAAATTCATCATCTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAAt                         >  2:800780/1‑81 (MQ=255)                                                             atcatcTTTGCCAAAGAAGAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGt                  >  2:800782/1‑79 (MQ=255)                                                                            gaagaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGc  >  1:800785/1‑80 (MQ=255)                                                                               |                                                                             ATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGC  >  NC_000913/4362072‑4362226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑