Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,402,976	G→A	P313P (CCG→CCA)	hflK →	regulator of FtsH protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,402,976	0	G	A	100.0%	104.5 / NA	31	P313P (CCG→CCA)	hflK	regulator of FtsH protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

GAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAA  >  NC_000913/4402899‑4403047                                                                              |                                                                        gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGaa                                                                      >  2:809644/1‑81 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGa                                                                       <  1:809600/80‑1 (MQ=255)      ggCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATaa                                                                 <  2:809601/81‑1 (MQ=255)       gcgcGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAAtata                                                                  >  2:809641/1‑79 (MQ=255)       gcgcGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATaaa                                                                <  2:809602/81‑1 (MQ=255)        cgcgTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAg                                                               <  2:809603/81‑1 (MQ=255)          cgtgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGcc                                                             <  2:809604/81‑1 (MQ=255)            tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCcgc                                                           >  1:809632/1‑81 (MQ=255)            tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCcgc                                                           <  2:809605/81‑1 (MQ=255)               gtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGcc                                                        <  1:809606/81‑1 (MQ=255)                   aaGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGaa                                                    <  1:809607/81‑1 (MQ=255)                    aGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGaaa                                                   <  2:809608/81‑1 (MQ=255)                         cAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACt                                              >  2:809650/1‑81 (MQ=255)                         cAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACt                                              <  2:809609/81‑1 (MQ=255)                          aGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTc                                             >  1:809649/1‑81 (MQ=255)                                  cTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGt                                     <  2:809610/81‑1 (MQ=255)                                  cTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGt                                     >  2:809637/1‑81 (MQ=255)                                     gAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTc                                    <  2:809611/79‑1 (MQ=255)                                     gAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTg                                  >  2:809639/1‑81 (MQ=255)                                      aaGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGt                                 <  2:809612/81‑1 (MQ=255)                                       aGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGta                                <  1:809613/81‑1 (MQ=255)                                          tCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTatatat                             >  2:809647/1‑81 (MQ=255)                                                tGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGAc                       >  2:809651/1‑81 (MQ=255)                                                tGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGAc                       >  2:809638/1‑81 (MQ=255)                                                   aGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGAt                    >  1:809654/1‑81 (MQ=255)                                                    gTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATg                   <  1:809614/81‑1 (MQ=255)                                                      ggCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATgg                  >  2:809640/1‑80 (MQ=255)                                                      ggCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATgg                  >  1:809646/1‑80 (MQ=255)                                                        cgcgcTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGaaa               <  1:809615/81‑1 (MQ=255)                                                               tGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAgtgt         <  2:809616/80‑1 (MQ=255)                                                                     aCTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTaa  <  2:809617/81‑1 (MQ=255)                                                                              |                                                                        GAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAA  >  NC_000913/4402899‑4403047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑