Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,837	C→A	E49D (GAG→GAT)	ytfE ←	iron‑sulfur cluster repair protein YtfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,837	0	C	A	100.0%	103.2 / NA	30	E49D (GAG→GAT)	ytfE	iron‑sulfur cluster repair protein YtfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/17);  total (13/17)

ATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATC  >  NC_000913/4431760‑4431908                                                                              |                                                                        atTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATc                                                                       >  2:815778/1‑80 (MQ=255)        ccAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCa                                                               <  1:815740/81‑1 (MQ=255)         cAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAg                                                              >  2:815774/1‑81 (MQ=255)             ggggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCt                                                          <  2:815741/81‑1 (MQ=255)                 cgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTAc                                                      <  1:815742/81‑1 (MQ=255)                 cgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTAc                                                      <  2:815743/81‑1 (MQ=255)                     aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgc                                                  >  1:815768/1‑81 (MQ=255)                      cGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgc                                                  <  2:815744/80‑1 (MQ=255)                       gCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccg                                                >  1:815784/1‑81 (MQ=255)                        ccAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccgc                                               <  1:815745/81‑1 (MQ=255)                         cAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccgcc                                              <  2:815746/81‑1 (MQ=255)                                tctcAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCcgcgc                                          <  2:815747/78‑1 (MQ=255)                                    aaTCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCa                                    <  2:815748/80‑1 (MQ=255)                                     aTCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGc                                  >  2:815767/1‑81 (MQ=255)                                        ggTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTc                               >  2:815785/1‑81 (MQ=255)                                         gttgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCt                              >  2:815783/1‑81 (MQ=255)                                         gttgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCt                              <  1:815749/81‑1 (MQ=255)                                              tCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTa                         >  1:815789/1‑81 (MQ=255)                                               cAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTa                         <  2:815750/80‑1 (MQ=255)                                                 gCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCg                      <  1:815751/81‑1 (MQ=255)                                                   gAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGc                     >  1:815819/1‑80 (MQ=255)                                                   gAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGc                     >  2:815799/1‑80 (MQ=255)                                                        ttGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcag                >  2:815790/1‑80 (MQ=255)                                                           ccAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag             <  1:815752/80‑1 (MQ=255)                                                                tCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATc        >  2:815791/1‑80 (MQ=255)                                                                 cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc       <  2:815753/80‑1 (MQ=255)                                                                 cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc       <  2:815754/80‑1 (MQ=255)                                                                 cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc       <  1:815755/80‑1 (MQ=255)                                                                    cTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCcatat   >  2:815795/1‑81 (MQ=255)                                                                       cAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCcatatc  <  2:815756/79‑1 (MQ=255)                                                                              |                                                                        ATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATC  >  NC_000913/4431760‑4431908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑