Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,613	T→A	I107I (ATT→ATA)	rihC →	ribonucleoside hydrolase RihC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,613	0	T	A	100.0%	144.7 / NA	41	I107I (ATT→ATA)	rihC	ribonucleoside hydrolase RihC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/17);  total (24/17)

CGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGT  >  NC_000913/27538‑27686                                                                            |                                                                          cGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGa                                                                      >  1:5414/1‑81 (MQ=255)  gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                     <  1:5379/81‑1 (MQ=255)  gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                     >  1:5416/1‑81 (MQ=255)  gAATCGGGAATGGCTGGCTACGACTTTGCTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                     >  2:5433/1‑81 (MQ=255)   aaTCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATg                                                                    <  1:5380/81‑1 (MQ=255)      cGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGccc                                                                 >  2:5413/1‑81 (MQ=255)       gggAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCt                                                                >  2:5429/1‑81 (MQ=255)       gggAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCt                                                                >  2:5426/1‑81 (MQ=255)        ggAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTg                                                               <  2:5381/81‑1 (MQ=255)           aTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATg                                                            <  2:5382/81‑1 (MQ=255)            tggctggcTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGc                                                           <  2:5383/81‑1 (MQ=255)                  gctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCAc                                                     <  2:5384/81‑1 (MQ=255)                   ctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCAc                                                     >  1:5438/1‑80 (MQ=255)                       gACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCagag                                                <  1:5385/81‑1 (MQ=255)                        aCTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGc                                               >  1:5441/1‑81 (MQ=255)                            tgttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTg                                            <  1:5386/80‑1 (MQ=255)                             gttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGt                                           >  2:5423/1‑80 (MQ=255)                              ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                          <  1:5389/80‑1 (MQ=255)                              ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                          <  2:5387/80‑1 (MQ=255)                              ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                          <  2:5388/80‑1 (MQ=255)                              ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTa                                         >  2:5434/1‑81 (MQ=255)                                gAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTAcc                                       >  1:5445/1‑81 (MQ=255)                                    acaACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtgg                                   >  2:5428/1‑81 (MQ=255)                                      aaCCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggtg                                 >  1:5431/1‑81 (MQ=255)                                      aaCCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggtg                                 >  1:5422/1‑81 (MQ=255)                                          gAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCa                             >  2:5442/1‑81 (MQ=255)                                            aaGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                           <  2:5390/81‑1 (MQ=255)                                            aaGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                           <  2:5392/81‑1 (MQ=255)                                            aaGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                           <  1:5391/81‑1 (MQ=255)                                            aaGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                           >  2:5436/1‑81 (MQ=255)                                                 gCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGccc                      <  2:5393/81‑1 (MQ=255)                                                 gCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGccc                      >  1:5456/1‑81 (MQ=255)                                                  cTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCg                     >  1:5452/1‑81 (MQ=255)                                                     gggATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGtt                   >  2:5440/1‑80 (MQ=255)                                                       gATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAAc                >  1:5435/1‑81 (MQ=255)                                                         tACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCa              <  1:5394/81‑1 (MQ=255)                                                          aCCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCaa             >  2:5437/1‑81 (MQ=255)                                                                gTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTgc       >  2:5454/1‑81 (MQ=255)                                                                    cTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt    <  1:5395/80‑1 (MQ=255)                                                                    cTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTg   >  2:5446/1‑81 (MQ=255)                                                                      ggCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGt  >  2:5439/1‑80 (MQ=255)                                                                            |                                                                          CGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGT  >  NC_000913/27538‑27686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑