Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,085	T→C	T108A (ACA→GCA)	idnD ←	L‑idonate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,085	0	T	C	100.0%	79.4 / NA	23	T108A (ACA→GCA)	idnD	L‑idonate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/17);  total (6/17)

TCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTT  >  NC_000913/4494009‑4494159                                                                             |                                                                           tCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGcaca                                                                         <  1:828223/80‑1 (MQ=255)     ccATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGAt                                                                    >  1:828262/1‑81 (MQ=255)      cATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGAtt                                                                   <  1:828224/81‑1 (MQ=255)             taACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGtt                                                             <  2:828225/80‑1 (MQ=255)               aCGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATg                                                          <  2:828226/81‑1 (MQ=255)                   gTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCa                                                      <  2:828227/81‑1 (MQ=255)                      aaaCCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATg                                                   <  1:828228/81‑1 (MQ=255)                          caccaTCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAg                                                >  2:828269/1‑80 (MQ=255)                                 aaCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGc                                         <  2:828229/80‑1 (MQ=255)                                  aCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAg                                       >  1:828267/1‑81 (MQ=255)                                    aTGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTg                                     <  2:828230/81‑1 (MQ=255)                                      gAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGAc                                   >  2:828258/1‑81 (MQ=255)                                       aGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGAcc                                  <  1:828231/81‑1 (MQ=255)                                         ggAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGc                                <  2:828232/81‑1 (MQ=255)                                                  tGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAg                       <  1:828233/81‑1 (MQ=255)                                                   ggCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGa                      >  1:828270/1‑81 (MQ=255)                                                    gCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTTACCGCACGGTTTAGAc                     <  1:828234/81‑1 (MQ=255)                                                          ccAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGAtt                <  1:828235/80‑1 (MQ=255)                                                              aaaaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATgg           <  2:828236/81‑1 (MQ=255)                                                              aaaaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATgg           >  2:828272/1‑81 (MQ=255)                                                                aaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCt         <  2:828237/81‑1 (MQ=255)                                                                   cGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTAcc      <  1:828238/81‑1 (MQ=255)                                                                      atatCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGtt   <  2:828239/81‑1 (MQ=255)                                                                       tatCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGttt  <  1:828240/81‑1 (MQ=255)                                                                             |                                                                           TCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTT  >  NC_000913/4494009‑4494159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑