Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,866	G→A	F284F (TTC→TTT)	fecR ←	regulator for fec operon, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,866	0	G	A	100.0%	100.7 / NA	29	F284F (TTC→TTT)	fecR	regulator for fec operon, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/11);  total (18/11)

GTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGC  >  NC_000913/4516788‑4516943                                                                               |                                                                              gTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGaaa                                                                             <  1:832638/81‑1 (MQ=255)  tACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAAc                                                                            <  2:832639/81‑1 (MQ=255)   aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACg                                                                           >  2:832668/1‑81 (MQ=255)   aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACg                                                                           >  2:832677/1‑81 (MQ=255)      gcgTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTcc                                                                        <  1:832640/81‑1 (MQ=255)        gTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCg                                                                      >  1:832667/1‑81 (MQ=255)                   aaTTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCa                                                            <  1:832641/80‑1 (MQ=255)                     ttttAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGcc                                                         <  2:832642/81‑1 (MQ=255)                            gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc                                                  >  1:832679/1‑81 (MQ=255)                            gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc                                                  >  1:832689/1‑81 (MQ=255)                            gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc                                                  <  2:832643/81‑1 (MQ=255)                               aaGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAcggc                                               <  1:832644/81‑1 (MQ=255)                                    tttGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGAt                                          >  2:832686/1‑81 (MQ=255)                                    tttGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGAt                                          <  2:832645/81‑1 (MQ=255)                                           gATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGATACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcg                                   >  1:832671/1‑81 (MQ=255)                                            aTAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcg                                   >  2:832675/1‑80 (MQ=255)                                               aCGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcgcag                                >  1:832678/1‑80 (MQ=255)                                                 gTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCAc                             >  1:832682/1‑81 (MQ=255)                                                    cAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc                          >  1:832699/1‑81 (MQ=255)                                                    cAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc                          >  1:832674/1‑81 (MQ=255)                                                        aTCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTg                      <  1:832646/81‑1 (MQ=255)                                                           gCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCgg                   >  2:832647/1‑81 (MQ=255)                                                            cATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCgg                   >  1:832684/1‑80 (MQ=255)                                                                  tATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg            <  2:832648/81‑1 (MQ=255)                                                                   aTTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGt           >  2:832692/1‑81 (MQ=255)                                                                    tttttCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGtt          <  1:832649/81‑1 (MQ=255)                                                                        tCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCg      >  1:832718/1‑81 (MQ=255)                                                                         cAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGt     >  1:832707/1‑81 (MQ=255)                                                                          aGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTg    >  1:832681/1‑81 (MQ=255)                                                                            cGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGc  <  2:832650/81‑1 (MQ=255)                                                                               |                                                                              GTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGC  >  NC_000913/4516788‑4516943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑