Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,579,647	C→A	G65V (GGC→GTC)	symE ←	toxic protein SymE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,579,647	0	C	A	100.0%	107.8 / NA	31	G65V (GGC→GTC)	symE	toxic protein SymE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/12);  total (19/12)

AGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGA  >  NC_000913/4579569‑4579722                                                                               |                                                                            aGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACt                                                                           >  2:844262/1‑81 (MQ=255) aGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACt                                                                           >  1:844250/1‑81 (MQ=255)  gTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACtt                                                                          <  2:844221/81‑1 (MQ=255)         cacCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCAc                                                                   >  1:844264/1‑81 (MQ=255)            cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt                                                                <  1:844222/81‑1 (MQ=255)              ggCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGa                                                              >  1:844267/1‑81 (MQ=255)                   aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc                                                          >  2:844260/1‑80 (MQ=255)                   aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc                                                          >  1:844259/1‑80 (MQ=255)                      gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc                                                      >  1:844261/1‑81 (MQ=255)                      gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc                                                      >  2:844272/1‑81 (MQ=255)                      gACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAcc                                                      <  2:844223/81‑1 (MQ=255)                                attCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAg                                            >  1:844263/2‑81 (MQ=255)                                   cGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg                                          <  1:844224/80‑1 (MQ=255)                                   cGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg                                          <  1:844225/80‑1 (MQ=255)                                    gCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGc                                        <  2:844226/81‑1 (MQ=255)                                          ctcGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAAc                                   <  2:844227/80‑1 (MQ=255)                                              gcggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcg                              >  2:844274/1‑81 (MQ=255)                                               cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg                             >  1:844283/1‑81 (MQ=255)                                               cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg                             <  2:844228/81‑1 (MQ=255)                                               cggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcgg                             >  1:844257/1‑81 (MQ=255)                                                     gtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCa                       <  1:844229/81‑1 (MQ=255)                                                       ggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGc                     >  2:844270/1‑81 (MQ=255)                                                         ttGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCa                   >  1:844265/1‑81 (MQ=255)                                                          tGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCAc                  >  2:844268/1‑81 (MQ=255)                                                           gggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACt                 >  2:844275/1‑81 (MQ=255)                                                            ggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTg                <  1:844230/81‑1 (MQ=255)                                                              cGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGAc              <  2:844231/81‑1 (MQ=255)                                                                gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt            >  1:844280/1‑81 (MQ=255)                                                                   aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt          <  2:844232/80‑1 (MQ=255)                                                                   aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt          >  1:844269/1‑80 (MQ=255)                                                                          tACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGa  >  1:844276/1‑81 (MQ=255)                                                                               |                                                                            AGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGA  >  NC_000913/4579569‑4579722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑