Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,596,634	C→T	A215A (GCC→GCT)	lgoD →	L‑galactonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,596,634	0	C	T	100.0%	84.4 / NA	25	A215A (GCC→GCT)	lgoD	L‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

GTGGCGGATACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAAT  >  NC_000913/4596560‑4596709                                                                           |                                                                            gtggCGGATACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGAt                                                                       <  2:847582/81‑1 (MQ=255)    gCGGATACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGAtttt                                                                    >  1:847633/1‑81 (MQ=255)    gCGGATACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGAtttt                                                                    <  2:847583/81‑1 (MQ=255)        aTACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGAcg                                                                >  1:847632/1‑81 (MQ=255)        aTACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGAcg                                                                >  1:847635/1‑81 (MQ=255)          aCCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGAcgcg                                                              >  1:847617/1‑81 (MQ=255)            cAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCa                                                            <  2:847584/81‑1 (MQ=255)             aGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAg                                                           <  2:847585/81‑1 (MQ=255)                ccGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTa                                                        >  1:847625/1‑81 (MQ=255)                       gcCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGgcgc                                                 <  1:847587/81‑1 (MQ=255)                       gcCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGgcgc                                                 <  2:847586/81‑1 (MQ=255)                              aCATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTtgg                                          <  2:847588/81‑1 (MQ=255)                                       aaCGCGTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCt                                 >  1:847647/1‑81 (MQ=255)                                            gTCTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGgcg                             >  2:847640/1‑80 (MQ=255)                                              cTGGAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGgcg                             >  2:847630/1‑78 (MQ=255)                                                ggAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGa                         >  1:847631/1‑80 (MQ=255)                                                 gAATTACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGa                         >  2:847638/1‑79 (MQ=255)                                                    ttACCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGt                     >  1:847639/1‑80 (MQ=255)                                                      aCCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTg                    <  2:847589/79‑1 (MQ=255)                                                      aCCTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGa                   <  1:847590/80‑1 (MQ=255)                                                       ccTTTACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGAt                  <  2:847591/80‑1 (MQ=255)                                                          ttACTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGAc              >  2:847627/1‑81 (MQ=255)                                                             cTGGACCCGTCAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGAcgcg           <  2:847592/81‑1 (MQ=255)                                                                     gTCAGCTGAAGATTTTGACGCGCAGCAACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTaa   <  1:847593/81‑1 (MQ=255)                                                                       cAGCTGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAAt  >  2:847634/1‑80 (MQ=255)                                                                           |                                                                            GTGGCGGATACCAGTCCGGCGCGCCGTGAACATGTGGCAACGCGTCTGGAATTACCTTTACTGGACCCGTCAGCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAAT  >  NC_000913/4596560‑4596709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑