Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,598,412	G→A	R344C (CGT→TGT)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,598,412	0	G	A	100.0%	67.4 / NA	20	R344C (CGT→TGT)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

CTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGG  >  NC_000913/4598342‑4598483                                                                       |                                                                        cTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACATGTACAAGAGATaaaa                                                               <  2:847944/81‑1 (MQ=255)    cAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCa                                                            >  2:847971/1‑81 (MQ=255)          aCCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCggg                                                      >  2:847978/1‑81 (MQ=255)                  gAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATggg                                               >  2:847998/1‑80 (MQ=255)                   aaTGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGt                                              <  2:847945/80‑1 (MQ=255)                    aTGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTAt                                            <  1:847946/81‑1 (MQ=255)                    aTGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTAt                                            <  2:847947/81‑1 (MQ=255)                     tGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATc                                           <  1:847948/81‑1 (MQ=255)                        cTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGAc                                        <  2:847949/81‑1 (MQ=255)                        cTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGAc                                        >  2:847977/1‑81 (MQ=255)                                 tttACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGt                               <  2:847950/81‑1 (MQ=255)                                   tACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTa                              >  2:847972/1‑80 (MQ=255)                                   tACCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTa                              <  1:847951/80‑1 (MQ=255)                                    aCCGTCAAAATCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaa                             <  1:847952/80‑1 (MQ=255)                                          aaaaTCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGt                        >  1:847976/1‑79 (MQ=255)                                            aaTCATATTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTgag                     >  1:847980/1‑80 (MQ=255)                                                atatTTTTTGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCg                >  2:847982/1‑81 (MQ=255)                                                      tttGCGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAcc          >  2:847979/1‑81 (MQ=255)                                                       tttcGGTTACAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCt         >  2:847981/4‑81 (MQ=255)                                                               aCAGGTACAAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCggg  >  2:847953/1‑80 (MQ=255)                                                                       |                                                                        CTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGG  >  NC_000913/4598342‑4598483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑