Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	463,039	G→A	V375V (GTG→GTA)	ppiD →	periplasmic folding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	463,039	0	G	A	100.0%	100.2 / NA	29	V375V (GTG→GTA)	ppiD	periplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

ACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTC  >  NC_000913/462967‑463116                                                                         |                                                                              aCGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACg                                                                        >  1:89604/1‑80 (MQ=255)   gTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGaa                                                                      >  2:89598/1‑80 (MQ=255)       ggACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGc                                                                 >  1:89612/1‑81 (MQ=255)        gacgacATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGcc                                                                >  2:89610/1‑81 (MQ=255)        gacgacATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGcc                                                                >  1:89605/1‑81 (MQ=255)            acATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTc                                                             >  1:89607/1‑80 (MQ=255)                tCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGc                                                        >  1:89599/1‑81 (MQ=255)                 cagccagcGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCg                                                       >  1:89609/1‑81 (MQ=255)                    ccagcGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGtac                                                    <  2:89566/81‑1 (MQ=255)                       gcGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGtactac                                                 >  2:89611/1‑81 (MQ=255)                            aGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCt                                            <  1:89568/81‑1 (MQ=255)                            aGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCt                                            <  1:89567/81‑1 (MQ=255)                                  aTCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTgcag                                        >  2:89595/1‑79 (MQ=255)                                       tAGACGAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAg                                 >  1:89616/1‑81 (MQ=255)                                            gAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGc                            >  1:89623/1‑81 (MQ=255)                                            gAAGTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGc                            <  1:89569/81‑1 (MQ=255)                                               gTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGAt                         <  2:89570/81‑1 (MQ=255)                                               gTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGAt                         <  2:89571/81‑1 (MQ=255)                                               gTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGAt                         <  2:89573/81‑1 (MQ=255)                                               gTACGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGAt                         <  1:89572/81‑1 (MQ=255)                                                  cGTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGc                       <  1:89574/80‑1 (MQ=255)                                                   gTGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCgg                     <  2:89575/81‑1 (MQ=255)                                                    tGACGACATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGgc                    <  2:89576/81‑1 (MQ=255)                                                          cATTGCGGCGAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGgcaagcaa              >  2:89618/1‑81 (MQ=255)                                                               cggcgAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGaca         <  1:89577/81‑1 (MQ=255)                                                                ggcgAAAGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGacac        >  1:89627/1‑81 (MQ=255)                                                                    aaaGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAg    >  2:89621/1‑81 (MQ=255)                                                                     aaGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGt   >  2:89620/1‑81 (MQ=255)                                                                      aGTAAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGtc  <  2:89578/81‑1 (MQ=255)                                                                         |                                                                              ACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTC  >  NC_000913/462967‑463116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑