Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	471,299	C→T	Q222* (CAG→TAG)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	471,299	0	C	T	100.0%	130.1 / NA	37	Q222* (CAG→TAG)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/22);  total (15/22)

TGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCC  >  NC_000913/471222‑471374                                                                              |                                                                            tgCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTtag                                                                           >  2:91129/1‑80 (MQ=255)   cgcgcCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTtagcag                                                                        <  1:91077/81‑1 (MQ=255)     cgcCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGgc                                                                      <  2:91078/81‑1 (MQ=255)     cgcCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGgc                                                                      >  2:91120/1‑81 (MQ=255)     cgcCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGgc                                                                      >  2:91112/1‑81 (MQ=255)      gcCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGgcg                                                                     <  2:91079/81‑1 (MQ=255)       ccTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGgcgc                                                                    <  1:91080/81‑1 (MQ=255)             tGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGAttt                                                               <  1:91081/80‑1 (MQ=255)              ggCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTg                                                              <  1:91082/80‑1 (MQ=255)                 ggATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGa                                                          <  1:91083/81‑1 (MQ=255)                  gATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGaa                                                         <  2:91084/81‑1 (MQ=255)                  gATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGaa                                                         >  1:91137/1‑81 (MQ=255)                   atatCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAAc                                                        <  2:91085/81‑1 (MQ=255)                   atatCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAAc                                                        <  2:91086/81‑1 (MQ=255)                     atCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACg                                                       >  1:91123/1‑80 (MQ=255)                           gacgGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATgggg                                                >  1:91131/1‑81 (MQ=255)                             cgGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATgggg                                                >  1:91146/1‑79 (MQ=255)                                 tttAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGc                                           >  2:91140/1‑80 (MQ=255)                                     aCGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTc                                      <  2:91087/81‑1 (MQ=255)                                        aaaTCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGtt                                   <  1:91088/81‑1 (MQ=255)                                               caATGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACta                            >  1:91133/1‑81 (MQ=255)                                                aaTGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACtat                           <  2:91089/81‑1 (MQ=255)                                                aaTGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACta                            >  2:91126/1‑80 (MQ=255)                                                aaTGGCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACta                            >  2:91144/1‑80 (MQ=255)                                                    gCATGAGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATgg                       <  2:91090/81‑1 (MQ=255)                                                         aGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAg                   <  1:91092/80‑1 (MQ=255)                                                         aGCGTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAg                   <  1:91091/80‑1 (MQ=255)                                                            gTTATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATg               <  1:91093/81‑1 (MQ=255)                                                              tATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGc              >  1:91139/1‑80 (MQ=255)                                                              tATCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGc              >  1:91134/1‑80 (MQ=255)                                                                tCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCaaa           <  2:91094/81‑1 (MQ=255)                                                                tCCAGCAGTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCaa            <  1:91095/80‑1 (MQ=255)                                                                  cagcagTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAAc          <  2:91096/80‑1 (MQ=255)                                                                      agTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTgc     >  1:91148/1‑81 (MQ=255)                                                                      agTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTgc     >  2:91125/1‑81 (MQ=255)                                                                      agTTTCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTgc     >  2:91141/1‑81 (MQ=255)                                                                      agTTTCGTTAGAAGGCGCGATTTGGCGAACGTATAGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTgc     <  1:91097/81‑1 (MQ=255)                                                                        tttCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTgcgc   <  2:91098/81‑1 (MQ=255)                                                                          tCGTTAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGcc  <  1:91099/80‑1 (MQ=255)                                                                              |                                                                            TGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCC  >  NC_000913/471222‑471374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑