Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,469	C→T	P31S (CCG→TCG)	ybaY →	PF09619 family lipoprotein YbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,469	0	C	T	100.0%	97.3 / NA	28	P31S (CCG→TCG)	ybaY	PF09619 family lipoprotein YbaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/9);  total (19/9)

CACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCT  >  NC_000913/475391‑475541                                                                               |                                                                         cacaTGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCg                                                                        >  2:91941/1‑81 (MQ=255)       gCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCa                                                                  <  1:91918/81‑1 (MQ=255)         cAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCaaa                                                                >  1:91964/1‑81 (MQ=255)               tttAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTc                                                          >  2:91940/1‑81 (MQ=255)                 tAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCt                                                         <  1:91919/80‑1 (MQ=255)                     ggTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTAttt                                                     >  2:91950/1‑80 (MQ=255)                          cGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaa                                               >  1:91944/1‑81 (MQ=255)                           gATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaa                                               >  2:91951/1‑80 (MQ=255)                           gATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaa                                               >  1:91943/1‑80 (MQ=255)                                   tGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACaacaac                                      >  1:91954/1‑81 (MQ=255)                                   tGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACaacaac                                      >  1:91955/1‑81 (MQ=255)                                    ggcggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACaacaac                                      >  1:91947/1‑80 (MQ=255)                                       ggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGc                                  >  2:91959/1‑81 (MQ=255)                                        gcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCt                                 <  1:91920/81‑1 (MQ=255)                                             cgcAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCa                            <  2:91921/81‑1 (MQ=255)                                                   tAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAAcc                      <  2:91922/81‑1 (MQ=255)                                                      aaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGaa                   >  1:91949/1‑81 (MQ=255)                                                      aaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGaa                   >  2:91958/1‑81 (MQ=255)                                                         cgcgGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGt                <  1:91923/81‑1 (MQ=255)                                                           cgGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGtct              >  2:91946/1‑81 (MQ=255)                                                              atatTCAGACGCCAGCATCGGCTGC‑‑ATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGt         <  2:91924/81‑1 (MQ=255)                                                                atTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCgg          >  2:91957/1‑80 (MQ=255)                                                                  tCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTAc       >  2:91952/1‑81 (MQ=255)                                                                   cAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTAcc      >  1:91974/1‑81 (MQ=255)                                                                    aGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCg     <  2:91926/81‑1 (MQ=255)                                                                    aGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCg     >  1:91956/1‑81 (MQ=255)                                                                    aGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCg     <  1:91925/81‑1 (MQ=255)                                                                       cGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCt  >  1:91965/1‑81 (MQ=255)                                                                               |                                                                         CACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCT  >  NC_000913/475391‑475541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑