Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	558,633	A→G	N141S (AAC→AGC)	sfmA →	putative fimbrial protein SfmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	558,633	0	A	G	100.0%	27.4 / NA	9	N141S (AAC→AGC)	sfmA	putative fimbrial protein SfmA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/2);  total (7/2)

AGCAACGGGTGTCGGTATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATC  >  NC_000913/558556‑558705                                                                              |                                                                         aGCAACGGGTGTCGGTATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAaccagcca                                                                       <  1:107584/81‑1 (MQ=255)       gggTGTCGGTATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACtt                                                                 >  1:107612/1‑81 (MQ=255)        ggTGTCGGTATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACtt                                                                 >  2:107611/1‑80 (MQ=255)                 aTTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCgg                                                        >  1:107613/1‑80 (MQ=255)                          cTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGt                                               <  2:107585/80‑1 (MQ=255)                             gATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGttctt                                           >  2:107607/1‑81 (MQ=255)                                   aCATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGTTCTTCAtttt                                     >  2:107605/1‑81 (MQ=255)                                     aTCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTc                                   >  2:107615/1‑81 (MQ=255)                                               ctcAAACCTGATGGGAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTta                          <  2:107586/80‑1 (MQ=255)                                                           gggAATAGCTTCTCAACCAGCCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCgg              >  2:107617/1‑80 (MQ=255)                                                                         aaccagccAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATc  <  1:107587/78‑1 (MQ=255)                                                                              |                                                                         AGCAACGGGTGTCGGTATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATC  >  NC_000913/558556‑558705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑