Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,401	G→A	A52A (GCG→GCA)	kefF →	regulator of KefC‑mediated potassium transport and quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,401	0	G	A	100.0%	100.2 / NA	29	A52A (GCG→GCA)	kefF	regulator of KefC‑mediated potassium transport and quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

CTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGAT  >  NC_000913/47324‑47478                                                                              |                                                                              cTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTg                                                                            <  2:9406/81‑1 (MQ=255) cTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTg                                                                            >  1:9439/1‑81 (MQ=255)    gAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGtct                                                                         >  2:9438/1‑81 (MQ=255)    gAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGtct                                                                         <  1:9407/81‑1 (MQ=255)     aaGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGtctc                                                                        <  1:9408/81‑1 (MQ=255)        gCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTcgcg                                                                     >  1:9431/1‑81 (MQ=255)          gTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGcc                                                                   <  1:9409/81‑1 (MQ=255)          gTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGcc                                                                   >  2:9451/1‑81 (MQ=255)               aaTTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCt                                                              <  2:9410/81‑1 (MQ=255)               aaTTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCt                                                              >  2:9449/1‑81 (MQ=255)               aaTTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCt                                                              >  2:9442/1‑81 (MQ=255)                   cGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATc                                                          >  2:9444/1‑81 (MQ=255)                        tctTTATCAACGCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTc                                                       <  1:9411/79‑1 (MQ=255)                          tttATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCt                                                      <  1:9412/78‑1 (MQ=255)                            tATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTgg                                                    <  1:9413/78‑1 (MQ=255)                                 aCTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATcc                                            <  2:9414/81‑1 (MQ=255)                                 aCTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATcc                                            <  1:9415/81‑1 (MQ=255)                                     tATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATg                                        >  1:9455/1‑81 (MQ=255)                                         cTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGt                                    <  2:9416/81‑1 (MQ=255)                                           gACTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTgg                                  >  2:9452/1‑81 (MQ=255)                                            aCTTCAATATCGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTg                                   <  2:9417/79‑1 (MQ=255)                                                      cGATATTGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATtcc                       >  2:9448/1‑81 (MQ=255)                                                           ttGCCGCCGAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGc                  <  2:9418/81‑1 (MQ=255)                                                                 ccgAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGctcctc             >  2:9471/1‑80 (MQ=255)                                                                   gAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCaaa          <  1:9419/81‑1 (MQ=255)                                                                   gAGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCaaa          >  2:9466/1‑81 (MQ=255)                                                                    aGCAGGAGGCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAAc         <  1:9420/81‑1 (MQ=255)                                                                         gaggCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTgg    <  2:9421/81‑1 (MQ=255)                                                                           ggCACTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGAt  >  1:9460/1‑81 (MQ=255)                                                                              |                                                                              CTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGAT  >  NC_000913/47324‑47478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑