Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	602,184	G→A	E76K (GAA→AAA)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	602,184	0	G	A	100.0%	93.1 / NA	27	E76K (GAA→AAA)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/11);  total (16/11)

GGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCT  >  NC_000913/602111‑602259                                                                          |                                                                            ggCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAAtggt                                                                      >  2:114315/1‑81 (MQ=255)   cGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAAtggtgg                                                                    >  1:114316/1‑81 (MQ=255)    gggTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATggtggt                                                                   >  1:114330/1‑81 (MQ=255)      gTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGtt                                                                  >  1:114322/1‑80 (MQ=255)          ggCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgag                                                               >  2:114331/1‑79 (MQ=255)          ggCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgag                                                               >  1:114318/1‑79 (MQ=255)           gCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgaggag                                                            <  2:114288/81‑1 (MQ=255)           gCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgaggag                                                            <  1:114286/81‑1 (MQ=255)           gCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgaggag                                                            <  2:114287/81‑1 (MQ=255)            cTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGCTTATGCGCCAGCTTGGCAAAATGGTGGTTGGGGAGc                                                           <  2:114289/81‑1 (MQ=255)                aTTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCgg                                                        >  1:114313/1‑80 (MQ=255)                    cTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATc                                                    <  1:114290/80‑1 (MQ=255)                     tGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCg                                                  >  2:114321/1‑81 (MQ=255)                        gCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGtt                                               <  1:114291/81‑1 (MQ=255)                        gCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGtt                                               >  2:114310/1‑81 (MQ=255)                         cTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTc                                              <  2:114292/81‑1 (MQ=255)                            cGGCGTCGCCGGGATCATCGCTTTCCTGAATATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCa                                             <  2:114293/79‑1 (MQ=255)                              gCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTg                                         >  1:114328/1‑81 (MQ=255)                                         atcatcGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGcc                              >  1:114327/1‑81 (MQ=255)                                                cTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATaaa                        >  1:114332/1‑80 (MQ=255)                                                cTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAAt                       <  2:114294/81‑1 (MQ=255)                                                   tCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACt                    >  1:114326/1‑81 (MQ=255)                                                       gATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTggg                 >  1:114325/1‑80 (MQ=255)                                                             gcgcCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGtt          >  1:114329/1‑81 (MQ=255)                                                              cgcCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGttt         <  2:114295/81‑1 (MQ=255)                                                                  aGCTTGGCAAAATGG‑GGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCggg    >  1:114323/1‑81 (MQ=255)                                                                       ggCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCt  <  2:114296/79‑1 (MQ=255)                                                                          |                                                                            GGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCT  >  NC_000913/602111‑602259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑