Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	620,329	A→C	L287R (CTG→CGG)	fepG ←	ferric enterobactin ABC transporter membrane subunit FepG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	620,329	0	A	C	100.0%	84.9 / NA	24	L287R (CTG→CGG)	fepG	ferric enterobactin ABC transporter membrane subunit FepG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/12);  total (12/12)

CTGACGGTAACGACGCCAACCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACAGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATGTGCGGTGCGAC  >  NC_000913/620251‑620405                                                                               |                                                                             cTGACGGTAACGACGCCAACCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCgc                                                                            <  1:117561/81‑1 (MQ=255)   gACGGTAACGACGCCAACCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCgcgc                                                                          >  1:117596/1‑81 (MQ=255)          acgacgCCAACCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCAt                                                                   <  1:117562/81‑1 (MQ=255)                   aCCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCgccgcc                                                          >  1:117598/1‑81 (MQ=255)                     cGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTg                                                        >  2:117608/1‑81 (MQ=255)                     cGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTg                                                        >  2:117600/1‑81 (MQ=255)                         aGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGt                                                     >  1:117604/1‑80 (MQ=255)                              aTACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGccc                                               >  1:117599/1‑81 (MQ=255)                              aTACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGccc                                               >  1:117601/1‑81 (MQ=255)                                 cGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGccccc                                             <  1:117563/80‑1 (MQ=255)                                  ggCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCag                                           >  1:117612/1‑81 (MQ=255)                                         aCAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCgg                                    >  2:117603/1‑81 (MQ=255)                                          cAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGt                                   <  1:117565/81‑1 (MQ=255)                                          cAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGt                                   <  2:117564/81‑1 (MQ=255)                                                  gggCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTaa                           >  1:117609/1‑81 (MQ=255)                                                  gggCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTaa                           >  1:117611/1‑81 (MQ=255)                                                   ggCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTaa                           <  1:117566/80‑1 (MQ=255)                                                   ggCGCAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAAt                          <  1:117567/81‑1 (MQ=255)                                                       cAGAGATCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGt                      <  1:117568/81‑1 (MQ=255)                                                           gaTCGGCCGCCAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCggg                  <  2:117569/81‑1 (MQ=255)                                                                   gccAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATgtgc          <  1:117570/81‑1 (MQ=255)                                                                    ccAGCAGTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATgtgcg         <  1:117571/81‑1 (MQ=255)                                                                     cagcagTAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATgtgcgg        >  1:117606/1‑81 (MQ=255)                                                                           tAACCGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATGTGCGGTGCGAc  <  1:117572/81‑1 (MQ=255)                                                                               |                                                                             CTGACGGTAACGACGCCAACCGGAAGTTGATACGGCATAAACAGTTGTTGGGCGCAGAGATCGGCCGCCAGCAGTAACAGCGCCCCGCATAGCGCCGCCTGGGTTAGCCCCCAGCGAGCGGTGCCGCTAATGCGTCGGGCAATGTGCGGTGCGAC  >  NC_000913/620251‑620405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑