Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	643,214	A→T	K376I (AAA→ATA)	ybdR →	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	643,214	0	A	T	100.0%	86.2 / NA	25	K376I (AAA→ATA)	ybdR	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/13);  total (12/13)

GATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTC  >  NC_000913/643136‑643289                                                                               |                                                                            gATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATAc                                                                           <  1:121969/81‑1 (MQ=255)  aTTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACg                                                                          <  2:121970/81‑1 (MQ=255)   ttACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGt                                                                         <  1:121971/81‑1 (MQ=255)      cTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGa                                                                       <  1:121972/80‑1 (MQ=255)      cTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGa                                                                       <  2:121973/80‑1 (MQ=255)        gAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGaa                                                                      >  2:460165/1‑79 (MQ=255)         aaaCCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAagag                                                                   >  2:122004/1‑81 (MQ=255)            ccAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAgaggag                                                                >  1:121999/1‑81 (MQ=255)                aagaaATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGcc                                                            <  1:121974/81‑1 (MQ=255)                aagaaATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGcc                                                            >  1:121993/1‑81 (MQ=255)                aagaaATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGcc                                                            <  2:121975/81‑1 (MQ=255)                            ccaaCTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTc                                                <  2:121976/78‑1 (MQ=255)                                     tGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCg                                       >  2:122008/1‑81 (MQ=255)                                         gTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTg                                    >  2:122009/1‑80 (MQ=255)                                          tttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGca                                  >  2:121997/1‑81 (MQ=255)                                          tttGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGca                                  >  1:122012/1‑81 (MQ=255)                                            tGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGcac                                 <  1:121977/80‑1 (MQ=255)                                                  ggCCGCCCGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAgcgc                          <  2:121978/81‑1 (MQ=255)                                                        ccGGGGATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAggc                    >  1:122010/1‑81 (MQ=255)                                                            ggATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGgcggcg                >  2:122015/1‑81 (MQ=255)                                                            ggATATGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGgcggcg                <  2:421236/81‑1 (MQ=255)                                                              atatGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGgcggcg                >  2:122003/1‑79 (MQ=255)                                                               tatGAGATTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAg             <  2:121979/81‑1 (MQ=255)                                                                    gaTTTTCGAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGc        >  2:122011/1‑81 (MQ=255)                                                                           gAAATACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTc  <  1:121980/80‑1 (MQ=255)                                                                               |                                                                            GATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTC  >  NC_000913/643136‑643289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑