Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	645,865	C→T	C239Y (TGC→TAC)	citT ←	citrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	645,865	0	C	T	100.0%	100.7 / NA	29	C239Y (TGC→TAC)	citT	citrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

TGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACGTTTGGTGCC  >  NC_000913/645789‑645942                                                                             |                                                                              tgtgATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTagag                                                                           <  2:122461/81‑1 (MQ=255)          cGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACt                                                                   <  1:122462/80‑1 (MQ=255)          cGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTg                                                                  >  2:122494/1‑81 (MQ=255)             tttGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCaa                                                               <  1:122463/81‑1 (MQ=255)              ttGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAAc                                                              >  2:122489/1‑81 (MQ=255)                  aCAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCTGCAGGAAGTAGAGGAACCACTGCAACCAGc                                                          >  2:122500/1‑81 (MQ=255)                   cAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGc                                                          >  2:122507/1‑80 (MQ=255)                        cGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCt                                                    <  2:122464/81‑1 (MQ=255)                         gTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTg                                                   >  2:122504/1‑81 (MQ=255)                           aGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGa                                                 >  1:122495/1‑81 (MQ=255)                            ggAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGTAGTAGAGGAACCACTGCAACCAGCTAATCTGGa                                                 >  2:122506/1‑80 (MQ=255)                                aGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCg                                            <  1:122465/81‑1 (MQ=255)                                   cACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCa                                         <  2:122466/81‑1 (MQ=255)                                      ggCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAAtt                                      <  1:122467/81‑1 (MQ=255)                                       gcgcAATGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAAttt                                     <  1:122468/81‑1 (MQ=255)                                           aaTGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCt                                 >  1:122491/1‑81 (MQ=255)                                           aaTGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCt                                 >  2:122503/1‑81 (MQ=255)                                           aaTGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCt                                 >  1:122488/1‑81 (MQ=255)                                            aTGATAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCt                                 >  2:122493/1‑80 (MQ=255)                                                tAAGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGa                            <  2:122469/81‑1 (MQ=255)                                                 aaGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGaa                           >  2:122501/1‑81 (MQ=255)                                                  aGCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAAc                          >  1:122502/1‑81 (MQ=255)                                                   gCAAGATAACCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACt                         >  2:122490/1‑81 (MQ=255)                                                          aaCCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGAcc                  >  2:122497/1‑81 (MQ=255)                                                           aCCCCAACCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGAccc                 <  1:122470/81‑1 (MQ=255)                                                                 aCCGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACg           >  1:122515/1‑81 (MQ=255)                                                                  ccGGCAGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACGt          <  1:122471/81‑1 (MQ=255)                                                                       aGGAAGTAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACGTTTGGt     >  1:122498/1‑81 (MQ=255)                                                                          aaGTAGAGGAACCACTGCAACCAGCTAATCTAGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACGTTTGGTGcc  <  2:122472/81‑1 (MQ=255)                                                                             |                                                                              TGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAATTTTGCTGACGAACTCCAGACCCAGCACGTTTGGTGCC  >  NC_000913/645789‑645942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑