Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	713,939	C→T	L128L (CTG→TTG)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	713,939	0	C	T	100.0%	100.1 / NA	29	L128L (CTG→TTG)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

TGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATG  >  NC_000913/713862‑714014                                                                              |                                                                            tGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTgg                                                                          >  1:134594/1‑81 (MQ=255)   gCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGtt                                                                        <  1:134558/81‑1 (MQ=255)            ggCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATaa                                                                >  1:134559/1‑80 (MQ=255)             gCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATaaa                                                               >  1:134592/1‑80 (MQ=255)                   aTGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTg                                                        <  1:134560/81‑1 (MQ=255)                        aTTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACCATAAAAAAGGTGGCCCg                                                   >  1:134580/1‑81 (MQ=255)                        aTTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCg                                                   <  1:134561/81‑1 (MQ=255)                         ttGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGc                                                  >  2:134589/1‑81 (MQ=255)                           gtgCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTg                                                >  2:134586/1‑81 (MQ=255)                             gCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTgg                                               >  2:134587/1‑80 (MQ=255)                             gCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTgg                                               >  1:134581/1‑80 (MQ=255)                              cAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCa                                             <  1:134562/81‑1 (MQ=255)                               aGGAAAACAATGGCTTCACCCCGAAGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAg                                            <  1:134563/81‑1 (MQ=255)                                 gAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGAc                                          <  1:134564/81‑1 (MQ=255)                                  aaaaCAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACg                                         >  1:134630/1‑81 (MQ=255)                                  aaaaCAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACg                                         >  2:134591/1‑81 (MQ=255)                                         tGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGt                                  >  2:134588/1‑81 (MQ=255)                                             ttCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGAt                               >  1:134593/1‑80 (MQ=255)                                               cACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCAGGCAGACGGTATCGTGAtt                              <  1:134565/79‑1 (MQ=255)                                                     gACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTc                      >  2:134599/1‑81 (MQ=255)                                                        gCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCa                   >  1:134603/1‑81 (MQ=255)                                                         ccTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCAt                  <  1:134566/81‑1 (MQ=255)                                                          cTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATa                 <  1:134567/81‑1 (MQ=255)                                                            gCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAAc               >  2:134595/1‑81 (MQ=255)                                                              cGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAAccc             <  2:134568/81‑1 (MQ=255)                                                                   ccAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACccgccg         >  1:134600/1‑80 (MQ=255)                                                                    cAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGa       <  1:134569/81‑1 (MQ=255)                                                                         ccATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGAtg  <  1:134571/81‑1 (MQ=255)                                                                         ccATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGAt   <  2:134570/80‑1 (MQ=255)                                                                              |                                                                            TGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATG  >  NC_000913/713862‑714014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑