Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	720,905	A→G	intergenic (+65/+151)	ybfK → / ← kdpE	uncharacterized protein YbfK/DNA‑binding transcriptional activator KdpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	720,905	0	A	G	100.0%	23.2 / NA	8	intergenic (+65/+151)	ybfK/kdpE	uncharacterized protein YbfK/DNA‑binding transcriptional activator KdpE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/6);  total (2/6)

TCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAA  >  NC_000913/720835‑720958                                                                       |                                                      tCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCaa                                              <  2:135727/80‑1 (MQ=255)       tgtgAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAat                                       <  1:135728/81‑1 (MQ=255)              gaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAatttattt                                 >  1:135741/1‑80 (MQ=255)                                tcaaGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACt               <  2:135729/77‑1 (MQ=255)                                   aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGt           <  2:135730/81‑1 (MQ=255)                                       aaTACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAata       <  1:135731/81‑1 (MQ=255)                                        aTACGGTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAatat      <  2:135732/81‑1 (MQ=255)                                            ggTTGAACTGTGAAGGTGATCAATAAAGAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATaaaa  >  1:135742/1‑81 (MQ=255)                                                                       |                                                      TCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAA  >  NC_000913/720835‑720958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑