Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	734,251	C→T	pseudogene (32/1434 nt)	ybfO →	conserved protein, rhs‑like YbfO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	734,251	0	C	T	100.0%	18.2 / NA	10	pseudogene (32/1434 nt)	ybfO	conserved protein, rhs‑like YbfO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

AGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCG  >  NC_000913/734217‑734326                                   |                                                                            aGCATGTGGCCGGATAACCGTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGaa                                >  1:138467/1‑80 (MQ=21)     tgtgGCCGGATAACCGTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAAcc                            >  1:138460/1‑80 (MQ=21)        ggCCGGATAACCGTATCGCCCGTGACGTGCACTATCTTTACTGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGccc                        >  1:138505/1‑81 (MQ=11)         gCCGGATAACCGTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGAcc                        <  1:138441/80‑1 (MQ=21)            ggATAACCGTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCAt                    >  1:138481/1‑81 (MQ=18)             gATAACCGTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATc                   <  1:138442/81‑1 (MQ=18)                    gTATCGCCCGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAg            >  1:138470/1‑81 (MQ=18)                           ccGTGACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTAt     >  2:138471/1‑81 (MQ=18)                               gACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCg  >  1:138477/1‑80 (MQ=21)                               gACGTGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCg  >  2:138480/1‑80 (MQ=21)                                   |                                                                            AGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCG  >  NC_000913/734217‑734326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑