Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,748	C→T	A160V (GCA→GTA)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,748	0	C	T	100.0%	109.5 / NA	32	A160V (GCA→GTA)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

TAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTC  >  NC_000913/812673‑812822                                                                            |                                                                           tAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAgg                                                                       <  2:152063/81‑1 (MQ=255)   acacAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGt                                                                      <  1:152064/80‑1 (MQ=255)   acacAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTa                                                                     <  1:152065/81‑1 (MQ=255)       aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATa                                                                  >  2:152105/1‑80 (MQ=255)       aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATa                                                                  >  1:152099/1‑80 (MQ=255)           acaacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATacaaca                                                             <  2:152066/81‑1 (MQ=255)                 gCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCg                                                        >  1:152112/1‑80 (MQ=255)                 gCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCg                                                        >  2:152118/1‑80 (MQ=255)                  ccGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCg                                                        <  2:152067/79‑1 (MQ=255)                    ggTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGgact                                                    >  2:152119/1‑81 (MQ=255)                    ggTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGgact                                                    <  2:152068/81‑1 (MQ=255)                        aTACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGgactgact                                                >  1:152098/1‑81 (MQ=255)                          aCTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGActct                                              >  1:152108/1‑81 (MQ=255)                              gTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTgg                                            <  2:152069/79‑1 (MQ=255)                               tAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCgg                                         >  2:152120/1‑81 (MQ=255)                                             cTCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAAc                           >  2:152114/1‑81 (MQ=255)                                              tCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAAc                           >  2:152123/1‑80 (MQ=255)                                              tCGGCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAAc                           >  1:152115/1‑80 (MQ=255)                                                ggCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGa                         <  2:152071/80‑1 (MQ=255)                                                ggCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGAt                        <  2:152070/81‑1 (MQ=255)                                                ggCTGTATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGAt                        >  1:152107/1‑81 (MQ=255)                                                     tATTAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGtt                     <  2:152072/79‑1 (MQ=255)                                                        tAATCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGcc                >  1:152111/1‑81 (MQ=255)                                                          aTCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCt               <  2:152074/80‑1 (MQ=255)                                                          aTCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTc              <  2:152073/81‑1 (MQ=255)                                                           tCACGCGATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTcc             <  2:152075/81‑1 (MQ=255)                                                              cgcgATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCggg          <  2:152076/81‑1 (MQ=255)                                                              cgcgATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCggg          <  1:152077/81‑1 (MQ=255)                                                              cgcgATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCgg           >  2:152117/1‑80 (MQ=255)                                                                 gATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaa        >  2:152116/1‑80 (MQ=255)                                                                 gATGTTGACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaa        >  2:152121/1‑80 (MQ=255)                                                                       gACTGTACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTc  <  2:152078/80‑1 (MQ=255)                                                                            |                                                                           TAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTC  >  NC_000913/812673‑812822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑