Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,147	G→A	Q276* (CAG→TAG)	ybhG ←	HlyD_D23 family protein YbhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,147	0	G	A	100.0%	134.4 / NA	39	Q276* (CAG→TAG)	ybhG	HlyD_D23 family protein YbhG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

AGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGG  >  NC_000913/829069‑829214                                                                               |                                                                    aGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTAcc                                                                   >  2:155121/1‑81 (MQ=255)  gACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTAccc                                                                  >  2:155140/1‑81 (MQ=255)  gACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTAcc                                                                   <  2:155080/80‑1 (MQ=255)     gAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGt                                                                >  2:155155/1‑80 (MQ=255)     gAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGt                                                                >  2:155127/1‑80 (MQ=255)      aGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGa                                                              <  2:155081/81‑1 (MQ=255)               cGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCtt                                                      <  1:155082/80‑1 (MQ=255)                gCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGt                                                    >  1:155120/1‑81 (MQ=255)                  aGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTcc                                                  >  2:155145/1‑81 (MQ=255)                   gATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCg                                                 >  1:155135/1‑81 (MQ=255)                           gTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCa                                         >  1:155133/1‑81 (MQ=255)                            tttCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCAt                                        <  1:155083/81‑1 (MQ=255)                             ttCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATc                                       >  1:155139/1‑81 (MQ=255)                              tCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCg                                      >  1:155143/1‑81 (MQ=255)                               cGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCgg                                     >  2:155131/1‑81 (MQ=255)                                  cGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGtat                                  <  2:155084/81‑1 (MQ=255)                                   ggTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGtat                                  >  2:155146/1‑80 (MQ=255)                                   ggTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGtat                                  >  2:155132/1‑80 (MQ=255)                                    gTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATaa                                <  1:155085/81‑1 (MQ=255)                                       ttCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAag                              <  2:155086/80‑1 (MQ=255)                                           gggTAAATTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAgcagca                         >  2:155134/1‑81 (MQ=255)                                                aaTTCAGCAGTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACttt                     <  1:155087/80‑1 (MQ=255)                                                    cagcagTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTgcgc                 <  1:155088/80‑1 (MQ=255)                                                    cagcagTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGcc                >  1:155142/1‑81 (MQ=255)                                                     agcagTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGcc                <  2:155089/80‑1 (MQ=255)                                                      gcagTCGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGcccc              <  2:155090/81‑1 (MQ=255)                                                           cGGCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTg         >  2:155138/1‑81 (MQ=255)                                                            ggCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTgg        <  2:155091/81‑1 (MQ=255)                                                             gCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTggg       <  2:155093/81‑1 (MQ=255)                                                             gCGAAACGAAACCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTggg       <  2:155092/81‑1 (MQ=255)                                                               gaaacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGcc     <  1:155095/81‑1 (MQ=255)                                                               gaaacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGcc     <  1:155094/81‑1 (MQ=255)                                                               gaaacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGcc     >  2:155137/1‑81 (MQ=255)                                                                aaacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCt    <  1:155096/81‑1 (MQ=255)                                                                aaacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCt    >  2:155147/1‑81 (MQ=255)                                                                 aacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTg   <  2:155097/81‑1 (MQ=255)                                                                 aacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTg   <  2:155099/81‑1 (MQ=255)                                                                 aacgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTg   <  1:155098/81‑1 (MQ=255)                                                                  acgaaacCAATCTACCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTgg  <  2:155100/81‑1 (MQ=255)                                                                               |                                                                    AGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGG  >  NC_000913/829069‑829214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑