Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	895,153	G→A	G55S (GGC→AGC)	potG →	putrescine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	895,153	0	G	A	100.0%	121.5 / NA	36	G55S (GGC→AGC)	potG	putrescine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/21);  total (15/21)

CGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATG  >  NC_000913/895077‑895228                                                                             |                                                                            cGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCt                                                                          <  1:166316/80‑1 (MQ=255)   aTGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCtgtg                                                                       <  2:166317/81‑1 (MQ=255)         aaCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGt                                                                 <  1:166318/81‑1 (MQ=255)          aCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTc                                                                >  2:166372/1‑81 (MQ=255)          aCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTc                                                                >  1:166366/1‑81 (MQ=255)                ggTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACgct                                                          <  1:166319/81‑1 (MQ=255)                   ggATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACgctgct                                                       >  2:166363/1‑81 (MQ=255)                   ggATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACgctgct                                                       >  1:166360/1‑81 (MQ=255)                    gatgatGTCAGCCTGACCATCTACAAAGGTAAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACgctgct                                                       <  2:166320/80‑1 (MQ=255)                        atgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTa                                                  >  2:166361/1‑81 (MQ=255)                          gTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATg                                                >  2:166368/1‑81 (MQ=255)                             aGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTg                                             <  1:166321/81‑1 (MQ=255)                              gCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTgg                                            >  2:166377/1‑81 (MQ=255)                               ccTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGACGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGc                                           <  1:166322/81‑1 (MQ=255)                                    ccATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGtt                                      <  2:166323/81‑1 (MQ=255)                                    ccATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGtt                                      >  2:166374/1‑81 (MQ=255)                                      aTCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTc                                    <  1:166324/81‑1 (MQ=255)                                          aCAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaa                                 >  2:755099/1‑80 (MQ=255)                                           cAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCg                                   <  2:166325/77‑1 (MQ=255)                                           cAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaac                                <  2:166326/80‑1 (MQ=255)                                           cAAAGGGGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaac                                <  1:166327/80‑1 (MQ=255)                                             aaGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaacaac                             >  1:166362/1‑81 (MQ=255)                                              aGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAAcc                            >  2:166367/1‑81 (MQ=255)                                               ggTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCt                           >  2:166370/1‑81 (MQ=255)                                                gTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCtt                          >  1:166369/1‑81 (MQ=255)                                                   aaaTCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCt                        <  2:166329/80‑1 (MQ=255)                                                   aaaTCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCt                        <  2:166328/80‑1 (MQ=255)                                                    aaTCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGc                      <  2:166330/81‑1 (MQ=255)                                                    aaTCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGc                      <  1:166331/81‑1 (MQ=255)                                                     aTCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGcc                     <  1:166332/81‑1 (MQ=255)                                                           gcgcTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAg               <  2:166333/81‑1 (MQ=255)                                                             gctgctGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGAt             <  2:166334/81‑1 (MQ=255)                                                                 ctgGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAAt          <  2:166335/80‑1 (MQ=255)                                                                   gggCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCt       >  1:166371/1‑81 (MQ=255)                                                                     gcgcATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCtt      >  1:166365/1‑80 (MQ=255)                                                                        cATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATg  <  1:166336/81‑1 (MQ=255)                                                                             |                                                                            CGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATG  >  NC_000913/895077‑895228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑