Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,736	A→T	F593L (TTT→TTA)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,736	0	A	T	100.0%	168.9 / NA	44	F593L (TTT→TTA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/16);  total (28/16)

AACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC  >  NC_000913/3471658‑3471813                                                                               |                                                                              aaCCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTtaa                                                                             <  2:699031/81‑1 (MQ=255)   ccTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACg                                                                           <  2:699032/81‑1 (MQ=255)          gATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  1:699089/1‑80 (MQ=255)          gATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  2:699104/1‑80 (MQ=255)          gATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTT‑AAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  1:699074/1‑79 (MQ=255)           aTCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  1:699084/1‑79 (MQ=255)              ggCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCagag                                                                >  1:699087/1‑81 (MQ=255)                cTCAAGCAGAACTGGTTTCGCTTTCCTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCagag                                                                >  2:699086/1‑79 (MQ=255)                   aaGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTc                                                           <  2:699033/81‑1 (MQ=255)                    aGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTc                                                           >  1:699083/1‑80 (MQ=255)                    aGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTc                                                           >  2:699113/1‑80 (MQ=255)                      cAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAAc                                                        >  2:699102/1‑81 (MQ=255)                      cAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAAc                                                        <  1:699034/81‑1 (MQ=255)                       aGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACg                                                       >  2:699094/1‑81 (MQ=255)                         aaCTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTc                                                     <  2:699035/81‑1 (MQ=255)                           cTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCAt                                                   <  1:699036/81‑1 (MQ=255)                           cTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCAt                                                   >  2:699106/1‑81 (MQ=255)                            tGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATg                                                  >  1:699079/1‑81 (MQ=255)                            tGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATg                                                  >  1:699091/1‑81 (MQ=255)                                ttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACATCATGGt                                                >  1:699108/1‑79 (MQ=255)                                  cGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAg                                             >  1:699107/1‑80 (MQ=255)                                  cGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAg                                             <  2:699037/80‑1 (MQ=255)                                     tttCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAcc                                         >  1:699109/1‑81 (MQ=255)                                       tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        <  1:699038/80‑1 (MQ=255)                                       tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        >  1:699105/1‑80 (MQ=255)                                        cTTAAAGCCTTTTTTAAAGGCGATAGGAGCCGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGa                                       <  1:699039/80‑1 (MQ=255)                                        cTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCAAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        >  2:699118/1‑79 (MQ=255)                                         ttAAACCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAg                                     >  2:699100/1‑81 (MQ=255)                                            aaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg                                   >  2:699095/1‑80 (MQ=255)                                              gCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg                                   >  2:699080/1‑78 (MQ=255)                                              gCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAg                                <  2:699040/81‑1 (MQ=255)                                                   cttTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGa                            <  1:699041/80‑1 (MQ=255)                                                     ttAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATa                         >  1:699130/1‑81 (MQ=255)                                                      tAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAc                        >  1:699103/1‑81 (MQ=255)                                                       aaaGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAc                        <  2:699042/80‑1 (MQ=255)                                                          ggCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCAt                    >  1:699096/1‑81 (MQ=255)                                                           gCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATg                   >  2:699093/1‑81 (MQ=255)                                                             gATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTc                 >  2:699115/1‑81 (MQ=255)                                                              aTAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTct                <  2:699043/81‑1 (MQ=255)                                                                 gAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctact             <  2:699044/81‑1 (MQ=255)                                                                    gcagcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTAcc          <  1:699045/81‑1 (MQ=255)                                                                      agcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCgg        >  2:699120/1‑81 (MQ=255)                                                                      agcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCgg        >  2:699114/1‑81 (MQ=255)                                                                      agcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCgg        <  2:699046/81‑1 (MQ=255)                                                                        ccAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGt      <  1:699047/81‑1 (MQ=255)                                                                            ttttAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGcc  <  2:699048/81‑1 (MQ=255)                                                                               |                                                                              AACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC  >  NC_000913/3471658‑3471813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑