Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,972	T→A	Q208L (CAG→CTG)	potA ←	spermidine preferential ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,972	0	T	A	100.0%	140.0 / NA	36	Q208L (CAG→CTG)	potA	spermidine preferential ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/17);  total (19/17)

CACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAC  >  NC_000913/1184895‑1185047                                                                              |                                                                            cACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGt                                                                          >  2:227174/1‑81 (MQ=255)  aCGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGt                                                                          <  2:227136/80‑1 (MQ=255)    gcggcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGt                                                                       <  1:227137/81‑1 (MQ=255)      ggcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGt                                                                       >  2:227172/1‑79 (MQ=255)        cgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGt                                                                   <  2:227138/81‑1 (MQ=255)         gTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTc                                                                  <  1:227139/81‑1 (MQ=255)            ccGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTcaca                                                               <  2:227140/81‑1 (MQ=255)             cGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTcaca                                                               >  2:227176/1‑80 (MQ=255)             cGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACaa                                                              <  1:227141/81‑1 (MQ=255)              gTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACaaa                                                             <  2:227142/81‑1 (MQ=255)                 ttGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGAc                                                          >  1:227211/1‑81 (MQ=255)                           cGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATg                                                >  1:227185/1‑81 (MQ=255)                            gACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTTAGTCACAAAGACGAATGTAATGc                                               >  1:227188/1‑81 (MQ=255)                            gACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGc                                               <  1:227143/81‑1 (MQ=255)                              ccATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCa                                             <  2:227144/81‑1 (MQ=255)                                aTCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAg                                           >  1:227186/1‑81 (MQ=255)                                  cgcgCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCt                                         >  1:227184/1‑81 (MQ=255)                                    cgcATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTa                                       >  2:227179/1‑81 (MQ=255)                                      cATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACg                                     >  2:227191/1‑81 (MQ=255)                                       aTCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGc                                    >  1:227178/1‑81 (MQ=255)                                          accacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTg                                  >  2:227190/1‑80 (MQ=255)                                           ccacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGt                                 >  2:227197/1‑80 (MQ=255)                                             acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc                              <  1:227145/81‑1 (MQ=255)                                             acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc                              <  1:227146/81‑1 (MQ=255)                                             acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc                              >  2:227180/1‑81 (MQ=255)                                             acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc                              <  1:227147/81‑1 (MQ=255)                                              caATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACg                             <  2:227148/81‑1 (MQ=255)                                                      tCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCa                       <  2:227149/79‑1 (MQ=255)                                                         gACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCg                  >  1:227192/1‑81 (MQ=255)                                                          aCATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCg                  <  1:227150/80‑1 (MQ=255)                                                            aTGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTc               >  2:227170/1‑81 (MQ=255)                                                            aTGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTc               >  2:227193/1‑81 (MQ=255)                                                                  aGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCAtt         <  1:227151/81‑1 (MQ=255)                                                                      cttcttCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGc      <  2:227152/80‑1 (MQ=255)                                                                         cttcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAc  >  1:227201/1‑81 (MQ=255)                                                                         cttcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAc  >  1:227187/1‑81 (MQ=255)                                                                              |                                                                            CACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAC  >  NC_000913/1184895‑1185047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑