Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,770,180	T→C	N369N (AAT→AAC)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,770,180	0	T	C	100.0%	90.1 / NA	24	N369N (AAT→AAC)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

GTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCT  >  NC_000913/1770102‑1770249                                                                               |                                                                      gtgggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACaa                                                                     <  1:340501/81‑1 (MQ=255)       ggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAAtt                                                               >  1:340527/1‑81 (MQ=255)        gaagaaGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAAttt                                                              >  2:340537/1‑81 (MQ=255)          agaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTtct                                                            <  2:340502/81‑1 (MQ=255)           gaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTtct                                                            >  2:340528/1‑80 (MQ=255)             agTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                         >  1:340531/1‑81 (MQ=255)              gTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                         >  2:340529/1‑80 (MQ=255)                     cgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAggcggc                                                 >  2:340536/1‑81 (MQ=255)                        cgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGctct                                              <  1:340503/81‑1 (MQ=255)                          aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgc                                            >  1:340533/1‑81 (MQ=255)                                   ccGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGAtt                                   <  1:340504/81‑1 (MQ=255)                                       aagaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGtt                                >  1:340542/1‑80 (MQ=255)                                       aagaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTa                               <  2:340505/81‑1 (MQ=255)                                             ttCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccacc                         <  2:340506/81‑1 (MQ=255)                                                  gaagaaCTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGc                       <  1:340507/78‑1 (MQ=255)                                                   aagaaCTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAt                   <  1:340508/81‑1 (MQ=255)                                                       aCTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGat               <  1:340509/81‑1 (MQ=255)                                                       aCTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGa                >  2:340541/1‑80 (MQ=255)                                                              gAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgct        >  1:340549/1‑81 (MQ=255)                                                               aaaTCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgctt       <  1:340510/81‑1 (MQ=255)                                                                  tCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTtgcttgct    >  1:340540/1‑81 (MQ=255)                                                                  tCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTtgcttgct    <  1:340511/81‑1 (MQ=255)                                                                   cGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc   >  1:340546/1‑81 (MQ=255)                                                                    gagaAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctct  >  1:340548/1‑81 (MQ=255)                                                                               |                                                                      GTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCT  >  NC_000913/1770102‑1770249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑