Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,133	G→A	T5I (ACT→ATT)	csrA ←	carbon storage regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,133	0	G	A	100.0%	97.5 / NA	26	T5I (ACT→ATT)	csrA	carbon storage regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/18);  total (8/18)

GGTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAGTCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCCATCAT  >  NC_000913/2819062‑2819207                                                                        |                                                                           ggTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAAt                                                                     >  2:541798/1‑79 (MQ=255)   ttGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGc                                                                 <  1:541752/81‑1 (MQ=255)         tttACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCtt                                                           >  1:541795/1‑81 (MQ=255)          ttACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCttt                                                          >  2:541768/1‑81 (MQ=255)          ttACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCttt                                                          >  1:541790/1‑81 (MQ=255)           tACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCtt                                                           <  2:541753/79‑1 (MQ=255)              cccTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTc                                                      >  2:541791/1‑81 (MQ=255)                      cTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGa                                                 >  1:541796/1‑78 (MQ=255)                        gTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGAt                                            >  1:541797/1‑81 (MQ=255)                           aCGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTaa                                         <  1:541754/81‑1 (MQ=255)                           aCGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTa                                          <  1:541755/80‑1 (MQ=255)                            cGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTaaa                                        <  1:541756/81‑1 (MQ=255)                                          ccAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGtctct                          <  2:541757/81‑1 (MQ=255)                                            aaTCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTctctct                        <  2:541758/81‑1 (MQ=255)                                              tCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGt                      <  1:541760/81‑1 (MQ=255)                                              tCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGt                      <  1:541759/81‑1 (MQ=255)                                                aTGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTAt                    <  2:541761/81‑1 (MQ=255)                                                  gAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATcc                  >  1:541804/1‑81 (MQ=255)                                                           aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCAtt           <  2:541762/79‑1 (MQ=255)                                                           aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTa          <  2:541765/80‑1 (MQ=255)                                                           aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTAt         <  1:541764/81‑1 (MQ=255)                                                           aCCAACTCGACGAATCAGAACCAGCATTCTTTGCTCCTTGAAAGTTTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTAt         <  1:541763/81‑1 (MQ=255)                                                               aCTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCcat     <  2:541766/81‑1 (MQ=255)                                                                   gacgaATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCcatcat  <  1:541767/80‑1 (MQ=255)                                                                   gacgaATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCcatcat  <  1:541769/80‑1 (MQ=255)                                                                   gacgaATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCcatca   <  1:541768/79‑1 (MQ=255)                                                                        |                                                                           GGTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAGTCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCCATCAT  >  NC_000913/2819062‑2819207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑