| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,152 | 0 | A | G | 20.0% | 128.1 / 10.8 | 50 | intergenic (+135/‑63) | lldD/trmL | L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (35/5); new base G (0/10); total (35/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTT > NC_000913/3781073‑3781232 | gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAg < 1:714820/81‑1 (MQ=255) gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg > 1:714877/1‑80 (MQ=255) tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAgggg < 2:714821/80‑1 (MQ=255) tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAgggg > 2:714875/1‑80 (MQ=255) tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg < 2:714822/79‑1 (MQ=255) tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑a < 1:714823/81‑1 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑aa > 1:714887/1‑80 (MQ=255) aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAgg > 2:714872/1‑81 (MQ=255) cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCg > 1:714890/1‑81 (MQ=255) ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGcc < 2:714824/79‑1 (MQ=255) tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCAc > 2:714894/1‑81 (MQ=255) tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCAc > 2:714882/1‑81 (MQ=255) cACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACt > 1:714879/1‑81 (MQ=255) ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGc > 1:714910/1‑81 (MQ=255) agAACCGACCCCGCACTTTTTTTTTC‑CCCCCCCCCCCCCGGGGGGGGGGCCGGGGGTGGGGGA‑AAAAGGCCGCACTGCt < 2:714826/78‑1 (MQ=255) gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCAc > 2:714907/1‑81 (MQ=255) aagcGACCCCGCCTTTTTTTTTTCCCCCCCCCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCAcc < 2:714830/78‑1 (MQ=255) aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCAc > 1:714897/1‑80 (MQ=255) aaaCCACCCCCCCCTTTTTTTTTCCCCCCCCCCCCCCCGGGGGGGGGGGC‑GGGGGGGGGGGAAAAAGGCCGCACTGCTCAc < 1:714829/81‑1 (MQ=255) aaaCAACCCCGCATTTTTTTTTTCCCCCCCGCCCCCCGGGGGGGGGGGCG‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTCAcc < 2:714825/81‑1 (MQ=255) aCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCaa > 1:714918/1‑81 (MQ=255) gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt > 2:714884/1‑81 (MQ=255) gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt > 2:714906/1‑81 (MQ=255) gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt > 1:714886/1‑81 (MQ=255) gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt > 1:714883/1‑81 (MQ=255) cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtttt > 2:714892/1‑81 (MQ=255) ctctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTAt > 1:714902/1‑81 (MQ=255) ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc > 2:714880/1‑81 (MQ=255) ttttttCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg > 1:714921/1‑81 (MQ=255) ttttttC‑CCCCCCCCCCCCCGGGGGGGGGGCC‑GGGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt < 1:714832/80‑1 (MQ=255) ttttCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc > 2:714912/1‑81 (MQ=255) tttCCCCCCCCCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc < 1:714844/80‑1 (MQ=255) ttCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc > 2:714917/1‑81 (MQ=255) cccGCCCCCCGGGGGGGGGGGCC‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt < 2:714836/79‑1 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 2:714901/1‑81 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 1:714893/1‑81 (MQ=255) ccccTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg > 1:714905/1‑81 (MQ=255) ccccTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg > 1:714922/1‑81 (MQ=255) ccgggggggggggCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc < 2:714842/78‑1 (MQ=255) ccGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 1:714881/1‑79 (MQ=255) ccGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 1:714895/1‑79 (MQ=255) ccGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg > 1:714909/1‑79 (MQ=255) cGGGGGGAGGGCC‑GGGGTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc < 2:714833/79‑1 (MQ=255) ggAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt > 2:714908/1‑81 (MQ=255) gagGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc > 1:714904/1‑80 (MQ=255) gggCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa < 1:714835/81‑1 (MQ=255) gCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa > 1:714891/1‑80 (MQ=255) c‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt > 2:714903/1‑81 (MQ=255) ggggggggggG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc < 1:714837/81‑1 (MQ=255) ggggggggggA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc < 2:714838/81‑1 (MQ=255) ggggTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc > 2:714896/1‑81 (MQ=255) gAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTCAGGGGGCAGTATGCTAAACATCGTACt > 1:714899/1‑81 (MQ=255) aGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt > 1:714873/1‑81 (MQ=255) | GCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTT > NC_000913/3781073‑3781232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |