Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,736	A→T	F593L (TTT→TTA)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,736	0	A	T	100.0%	143.5 / NA	38	F593L (TTT→TTA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/17);  total (21/17)

TGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC  >  NC_000913/3471666‑3471813                                                                       |                                                                              tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  2:613383/1‑81 (MQ=255) tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt                                                                     >  1:613388/1‑81 (MQ=255)       gCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAgagg                                                               <  2:613332/81‑1 (MQ=255)          cAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGt                                                            >  2:613404/1‑81 (MQ=255)           aaGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCGGAGGAGTc                                                           <  2:613333/81‑1 (MQ=255)               aGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACg                                                       >  1:613392/1‑81 (MQ=255)                gAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGt                                                      >  2:613389/1‑81 (MQ=255)                gAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGt                                                      >  1:613407/1‑81 (MQ=255)                      gTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt                                                <  2:613335/81‑1 (MQ=255)                      gTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTAATGGt                                                <  1:613334/81‑1 (MQ=255)                       tttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTa                                               <  2:613336/81‑1 (MQ=255)                        ttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt                                                >  2:613378/1‑79 (MQ=255)                        ttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt                                                >  1:613399/1‑79 (MQ=255)                          cGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAg                                             <  1:613337/80‑1 (MQ=255)                           gCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGaa                                           <  2:613338/81‑1 (MQ=255)                           gCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGa                                            <  2:613339/80‑1 (MQ=255)                               tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAcc                                         <  2:613341/79‑1 (MQ=255)                               tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        <  2:613340/80‑1 (MQ=255)                               tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        >  1:613386/1‑80 (MQ=255)                                cTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg                                        >  1:613428/1‑79 (MQ=255)                                   aaaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAg                                     <  1:613342/79‑1 (MQ=255)                                   aaaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg                                   >  1:613395/1‑81 (MQ=255)                                    aaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg                                   >  2:613411/1‑80 (MQ=255)                                      gCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAg                                <  2:613343/81‑1 (MQ=255)                                          tcttTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACg                             >  1:613391/1‑80 (MQ=255)                                              tAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAAt                          <  1:613344/79‑1 (MQ=255)                                              tAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAc                        >  2:613410/1‑81 (MQ=255)                                               aaaGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAcc                       >  1:613402/1‑81 (MQ=255)                                                 aGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCa                     >  1:613400/1‑81 (MQ=255)                                                  ggCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCAt                    <  2:613345/81‑1 (MQ=255)                                                   gCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATg                   >  1:613397/1‑81 (MQ=255)                                                   gCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATg                   <  2:613346/81‑1 (MQ=255)                                                      aTAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTct                <  2:613347/81‑1 (MQ=255)                                                        aGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctac              >  1:613393/1‑81 (MQ=255)                                                         gAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctact             <  1:613348/81‑1 (MQ=255)                                                         gAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctac              >  2:613394/1‑80 (MQ=255)                                                              agcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCgg        >  2:613398/1‑81 (MQ=255)                                                                 cAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCTATGTCTACTACCggg       >  1:613425/1‑79 (MQ=255)                                                                    ttttAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGcc  <  1:613349/81‑1 (MQ=255)                                                                    ttttAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGcc  <  1:613350/81‑1 (MQ=255)                                                                       |                                                                              TGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC  >  NC_000913/3471666‑3471813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑