Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,770,180	T→C	N369N (AAT→AAC)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,770,180	0	T	C	100.0%	64.8 / NA	18	N369N (AAT→AAC)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/5);  total (13/5)

AGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCC  >  NC_000913/1770111‑1770253                                                                      |                                                                          agaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTtct                                                                >  1:286478/1‑81 (MQ=255)     gTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                             >  2:286488/1‑80 (MQ=255)      tCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                             >  1:286496/1‑79 (MQ=255)         cgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAggc                                                        >  1:286486/1‑81 (MQ=255)          gccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAggcg                                                       >  1:286487/1‑81 (MQ=255)                 aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgc                                                >  1:286477/1‑81 (MQ=255)                 aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgc                                                >  2:286475/1‑81 (MQ=255)                   ggACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgccg                                              >  1:286481/1‑81 (MQ=255)                       cAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTg                                          <  2:286452/81‑1 (MQ=255)                              aagaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGtt                                    >  2:286484/1‑80 (MQ=255)                                    ttCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccacc                             <  1:286453/81‑1 (MQ=255)                                      ctcGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGc                           >  2:286483/1‑81 (MQ=255)                                                    cGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc             <  1:286455/81‑1 (MQ=255)                                                    cGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc             <  1:286454/81‑1 (MQ=255)                                                           gagaAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctct      >  2:286480/1‑81 (MQ=255)                                                              aaaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc   >  1:286494/1‑81 (MQ=255)                                                              aaaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc   >  2:286485/1‑81 (MQ=255)                                                               aaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc  <  2:286456/81‑1 (MQ=255)                                                                      |                                                                          AGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCC  >  NC_000913/1770111‑1770253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑