Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,133	G→A	T5I (ACT→ATT)	csrA ←	carbon storage regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,133	0	G	A	100.0%	93.1 / NA	25	T5I (ACT→ATT)	csrA	carbon storage regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/21);  total (4/21)

GTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAGTCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCCATCATAT  >  NC_000913/2819063‑2819209                                                                       |                                                                             gTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATc                                                                      <  2:458304/79‑1 (MQ=255)     cccTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCAtt                                                                <  1:458305/81‑1 (MQ=255)              ccTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTcc                                                       <  1:458306/81‑1 (MQ=255)                    aCTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGa                                                   >  2:458345/1‑79 (MQ=255)                        tCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGAtt                                             <  2:458307/81‑1 (MQ=255)                          aCGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTa                                            >  2:458338/1‑80 (MQ=255)                          aCGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTa                                            >  1:458341/1‑80 (MQ=255)                           cGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTaaa                                          <  2:458308/81‑1 (MQ=255)                           cGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTaaa                                          <  1:458309/81‑1 (MQ=255)                             gTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAag                                        <  2:458310/81‑1 (MQ=255)                                aCCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAagag                                      <  1:458311/80‑1 (MQ=255)                                 ccTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTc                                    <  1:458312/81‑1 (MQ=255)                                     aTCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGt                                <  2:458313/81‑1 (MQ=255)                                      tCCCCAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGtc                               <  2:458314/81‑1 (MQ=255)                                         ccAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGtctct                            <  2:458315/81‑1 (MQ=255)                                          cAATCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGtctctc                           <  2:458316/81‑1 (MQ=255)                                           aaTCATGAGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTctctct                          <  1:458317/81‑1 (MQ=255)                                                  aGGGTCTCACCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATccc                   <  1:458318/81‑1 (MQ=255)                                                          aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCAtt             <  2:458321/79‑1 (MQ=255)                                                          aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCAt              <  1:458319/78‑1 (MQ=255)                                                          aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTAt           <  1:458320/81‑1 (MQ=255)                                                          aCCAACTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTAt           <  2:458322/81‑1 (MQ=255)                                                             aaCTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCca        >  1:458344/1‑81 (MQ=255)                                                              aCTCGACGAATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCcat       <  1:458323/81‑1 (MQ=255)                                                                   acgaATCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCCATCatat  <  2:458324/81‑1 (MQ=255)                                                                       |                                                                             GTTGCCCTTTACCCCTAAAACTGTCACGGTGACCTCATCCCCAATCATGAGGGTCTCACCAACTCGACGAGTCAGAATCAGCATTCTTTGCTCCTTGAAAGATTAAAAGAGTCGGGTCTCTCTGTATCCCGGCATTATCCATCATAT  >  NC_000913/2819063‑2819209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑