Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,041,634 A→C T108P (ACC→CCC)  ygfZ → iron‑sulfur cluster repair protein, plumbagin resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,6340AC99.5% 800.7 / ‑4.7 211T108P (ACC→CCC) ygfZiron‑sulfur cluster repair protein, plumbagin resistance
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (103/106);  minor base T (0/1);  total (104/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 212 total aligned reads displayed.

GTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAG  >  NC_000913/3041537‑3041730
                                                                                                 |                                                                                                
gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGccc                                                                                                <  1:2737847/100‑1 (MQ=255)
 ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCa                                                                                               <  1:3516663/100‑1 (MQ=255)
  tCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCAt                                                                                              >  1:3817689/1‑100 (MQ=255)
      cgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcgcg                                                                                          >  1:4682985/1‑100 (MQ=255)
          ggCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAg                                                                                      >  1:3296045/1‑100 (MQ=255)
            cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCa                                                                                               >  2:1862573/1‑89 (MQ=255)
                 gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcgcg                                                                                          >  2:2272706/1‑89 (MQ=255)
                  cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAg                                                                              <  1:613242/100‑1 (MQ=255)
                   tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGcc                                                                                        <  2:3566503/89‑1 (MQ=255)
                    ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            <  1:4527036/100‑1 (MQ=255)
                    ttGCATGGATTGAACGGCGCAGCGTCCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            <  1:3917876/100‑1 (MQ=255)
                      gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgtg                                                                          >  1:4554038/1‑100 (MQ=255)
                       cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgtgt                                                                         >  1:5568007/1‑100 (MQ=255)
                       cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgtgt                                                                         >  1:5083816/1‑100 (MQ=255)
                         tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgc                                                                       >  1:2047634/1‑100 (MQ=255)
                         tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgc                                                                       <  1:1576323/100‑1 (MQ=255)
                         tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgc                                                                       <  1:4034144/100‑1 (MQ=255)
                         tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgc                                                                       <  1:1169879/100‑1 (MQ=255)
                            aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgctgc                                                                    <  1:2810924/100‑1 (MQ=255)
                            aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTAtgctgc                                                                    >  1:881152/1‑100 (MQ=255)
                             ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAg                                                                              >  2:4609456/1‑89 (MQ=255)
                              tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacg                                                                                   <  2:527036/83‑1 (MQ=255)
                              tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGc                                                                             >  2:2912008/1‑89 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            >  2:4911157/1‑89 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            >  2:907760/1‑89 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            >  2:4901440/1‑89 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            <  2:4982076/89‑1 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            >  2:460087/1‑89 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg                                                                            <  2:408132/89‑1 (MQ=255)
                               gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                 >  1:2425094/1‑100 (MQ=255)
                                aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg                                                                >  1:5356317/1‑100 (MQ=255)
                                aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg                                                                >  1:217353/1‑100 (MQ=255)
                                  cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                              >  1:1291626/1‑100 (MQ=255)
                                   ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg                                                                        <  2:2069448/89‑1 (MQ=255)
                                   ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt                                                             >  1:1569748/1‑100 (MQ=255)
                                   ggCGCAGCGTGCGTGAACCGCAGCTGACAGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt                                                             >  1:5279166/1‑100 (MQ=255)
                                    gcgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgc                                                                       <  2:1172809/89‑1 (MQ=255)
                                     cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgct                                                                      <  2:5421257/89‑1 (MQ=255)
                                      gcAGCGTGCGTGAACCGCAGCTGACTGCACTGAAAAAATATGCAGTATTCTCTAAAGTGCCAATCGCGCCAGACGACGAGCGTGtgctg                                                                     >  2:310997/1‑89 (MQ=255)
                                       cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                         <  1:1910483/100‑1 (MQ=255)
                                        aGCGTGTGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTgctgct                                                                   >  2:3277534/1‑89 (MQ=255)
                                          cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                      <  1:325597/100‑1 (MQ=255)
                                            tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                               >  2:2974239/1‑89 (MQ=255)
                                              cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtgt                                                             <  2:1294531/89‑1 (MQ=255)
                                              cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                  <  1:4735186/100‑1 (MQ=255)
                                              cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                  <  1:1508178/100‑1 (MQ=255)
                                               gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                 <  1:3772184/100‑1 (MQ=255)
                                                 gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                          >  2:834245/1‑89 (MQ=255)
                                                 gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                          >  2:265414/1‑89 (MQ=255)
                                                   aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                        >  2:1808037/1‑89 (MQ=255)
                                                   aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                             <  1:3857686/100‑1 (MQ=255)
                                                   aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                             <  1:2309067/100‑1 (MQ=255)
                                                    ccGCAGCTGACTGAAATGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                       <  2:1397736/89‑1 (MQ=255)
                                                     cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                      <  2:2371621/89‑1 (MQ=255)
                                                     cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                      <  2:1564607/89‑1 (MQ=255)
                                                       cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                    <  2:3695148/89‑1 (MQ=255)
                                                          ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                 <  2:3635247/89‑1 (MQ=255)
                                                          ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                 <  2:3361565/89‑1 (MQ=255)
                                                           tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                >  2:2230416/1‑89 (MQ=255)
                                                            gactgaACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                    <  1:4929981/100‑1 (MQ=255)
                                                             actgaactgaAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc                                   <  1:4839890/100‑1 (MQ=255)
                                                              ctgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                             <  2:608269/89‑1 (MQ=255)
                                                               tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                 <  1:2261962/100‑1 (MQ=255)
                                                                gaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcg                                           >  2:2487375/1‑89 (MQ=255)
                                                                gaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                <  1:2134617/100‑1 (MQ=255)
                                                                gaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                <  1:5257054/100‑1 (MQ=255)
                                                                 aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgc                                          >  2:482047/1‑89 (MQ=255)
                                                                 aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                               <  1:2795149/100‑1 (MQ=255)
                                                                  actgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                              <  1:4388697/100‑1 (MQ=255)
                                                                  actgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                              >  1:4066144/1‑100 (MQ=255)
                                                                   ctgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                        >  2:4258917/1‑89 (MQ=255)
                                                                   ctgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                        >  2:3143468/1‑89 (MQ=255)
                                                                     gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                      >  2:1779736/1‑89 (MQ=255)
                                                                       aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                    >  2:624717/1‑89 (MQ=255)
                                                                       aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                         >  1:41535/1‑100 (MQ=255)
                                                                         aaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                  <  2:2734448/89‑1 (MQ=255)
                                                                            tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACTAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                    <  1:707198/100‑1 (MQ=255)
                                                                            tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                            <  2:5524199/60‑1 (MQ=255)
                                                                            tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                            >  1:5524200/1‑60 (MQ=255)
                                                                            tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                               <  2:725194/89‑1 (MQ=255)
                                                                            tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                               <  2:928728/89‑1 (MQ=255)
                                                                             atGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                              >  2:387220/1‑89 (MQ=255)
                                                                              tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                             <  2:2796711/89‑1 (MQ=255)
                                                                               gCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCg                 <  1:5197924/100‑1 (MQ=255)
                                                                                cGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                           <  2:5034401/89‑1 (MQ=255)
                                                                                 ggTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                          <  2:2767367/89‑1 (MQ=255)
                                                                                    aTTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTg            <  1:1967664/100‑1 (MQ=255)
                                                                                     ttCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                      <  2:4457210/89‑1 (MQ=255)
                                                                                     ttCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                      <  2:341913/89‑1 (MQ=255)
                                                                                     ttCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc           <  1:2972113/100‑1 (MQ=255)
                                                                                      tctcTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                     >  2:4372656/1‑89 (MQ=255)
                                                                                       ctctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                    >  2:1995669/1‑89 (MQ=255)
                                                                                        tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg                   <  2:4737408/89‑1 (MQ=255)
                                                                                        tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt        >  1:3418809/1‑100 (MQ=255)
                                                                                         ctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc                  <  2:4417016/89‑1 (MQ=255)
                                                                                         ctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc                  >  2:1622383/1‑89 (MQ=255)
                                                                                         ctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc       <  1:3877351/100‑1 (MQ=255)
                                                                                           aaaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa                >  2:666923/1‑89 (MQ=255)
                                                                                             aGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc              <  2:388956/89‑1 (MQ=255)
                                                                                             aGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa   <  1:5033324/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAG  >  NC_000913/3041537‑3041730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: