Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,152,433 A→G S14P (TCG→CCG)  yegK ← ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,152,4330AG99.3% 486.0 / NA 137S14P (TCG→CCG) yegKser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (81/55);  minor base C (0/1);  total (81/56)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 140 total aligned reads displayed.

TGACGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGT  >  NC_000913/2152286‑2152580
                                                                                                                                                   |                                                                                                                                                   
tGACTGCTAGCATCGCGCCTTCGCCACCCGGCGAAACAGTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGCGCGCCGt                                                                                                                                                  <  2:218344/151‑1 (MQ=255)
   cGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACCATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCCTCCTGACAAGGCAAGTCCGCGCTGATGTCCGGCGTTcc                                                                                                                                               >  2:2258854/1‑151 (MQ=255)
   cGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTcc                                                                                                                                               >  2:1210956/1‑151 (MQ=255)
   cGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTcc                                                                                                                                               <  1:1464640/151‑1 (MQ=255)
   cGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTcc                                                                                                                                               >  1:2437786/1‑151 (MQ=255)
     gCGAGCATCGCGCCTCCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCaa                                                                                                                                             >  2:1073022/1‑151 (MQ=255)
      cGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAAc                                                                                                                                            <  1:464652/151‑1 (MQ=255)
         gCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAAGCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGACGGGCGGCGTTCCCACGGt                                                                                                                                         >  2:369481/1‑151 (MQ=255)
          cATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGtt                                                                                                                                        <  2:801174/151‑1 (MQ=255)
           aTCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTg                                                                                                                                       >  2:951021/1‑151 (MQ=255)
             cgcgCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGAt                                                                                                                                     <  1:1838848/151‑1 (MQ=255)
             cgcgCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGAt                                                                                                                                     <  2:2437760/151‑1 (MQ=255)
                 ccTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCAt                                                                                                                                 <  2:1630087/151‑1 (MQ=255)
                 ccTTCGCCACCCTGCGAGAAACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCAt                                                                                                                                 >  1:2376341/1‑151 (MQ=255)
                  cTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATa                                                                                                                                >  1:122714/1‑151 (MQ=255)
                  cTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATa                                                                                                                                >  2:1587867/1‑151 (MQ=255)
                  cTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGAGCTGATGTGCGGCGTTCCAACGGTTGATGCATa                                                                                                                                >  1:1609388/1‑151 (MQ=255)
                    tCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGa                                                                                                                              >  2:1358820/1‑151 (MQ=255)
                     cGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGAc                                                                                                                             <  1:842795/151‑1 (MQ=255)
                      gCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGAcc                                                                                                                            <  2:1897440/151‑1 (MQ=255)
                       ccACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCa                                                                                                                           >  1:142436/1‑151 (MQ=255)
                       ccACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCa                                                                                                                           >  2:378137/1‑151 (MQ=255)
                        cACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAg                                                                                                                          >  1:2041928/1‑151 (MQ=255)
                          cccTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGAAAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGAc                                                                                                                        <  1:2326759/151‑1 (MQ=255)
                               cGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAg                                                                                                                   >  1:2414960/1‑151 (MQ=255)
                                 agaCACTGCCTGCGCCATCAGCGACAAATACCGACAATAAGGGCGGCTGGTCATTTAACCAGGCAATCTGCAGCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGCTGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCt                                                                                                                 >  2:1307462/1‑151 (MQ=255)
                                  gaCACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTc                                                                                                                <  2:52907/151‑1 (MQ=255)
                                    cacTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCGTTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGCTGTGCGGCGTTCCAACGGTCGATGCATAGACCAGACGCCAGCGcac                                                                                                              >  2:948066/1‑151 (MQ=255)
                                    cacTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTcac                                                                                                              >  1:2563221/1‑151 (MQ=255)
                                      ctgcctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCacac                                                                                                            <  1:1933695/151‑1 (MQ=255)
                                      ctgcctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCacac                                                                                                            >  1:403145/1‑151 (MQ=255)
                                         cctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACCGTTGATGCATAGACCAGACGCCAGCTCACACTGa                                                                                                         >  1:761081/1‑151 (MQ=255)
                                              gcCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTcc                                                                                                    >  2:1100566/1‑151 (MQ=255)
                                                  tCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGcc                                                                                                >  2:1665649/1‑151 (MQ=255)
                                                    aGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCtt                                                                                              >  2:2047734/1‑151 (MQ=255)
                                                         cAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGCTGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGgc                                                                                         >  2:732483/1‑151 (MQ=255)
                                                             tACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCtt                                                                                     >  2:32751/1‑151 (MQ=255)
                                                              aCCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTc                                                                                    <  2:359504/151‑1 (MQ=255)
                                                               ccGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTcc                                                                                   <  1:1038183/151‑1 (MQ=255)
                                                               ccGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTcc                                                                                   <  1:1793350/151‑1 (MQ=255)
                                                                cGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCGGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGATCACACTGTCGTCCAGCCTTTTGGCGCTTCCa                                                                                  >  2:2368161/1‑151 (MQ=255)
                                                                 gACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAg                                                                                 >  2:637561/1‑151 (MQ=255)
                                                                  aCAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGc                                                                                <  1:281358/151‑1 (MQ=255)
                                                                    aataatGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGcac                                                                              <  1:1611499/151‑1 (MQ=255)
                                                                        atGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACt                                                                          >  2:2305767/1‑151 (MQ=255)
                                                                            ctgctgGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCg                                                                      >  1:2527352/1‑151 (MQ=255)
                                                                                 ggTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTccc                                                                 >  1:1261135/1‑151 (MQ=255)
                                                                                  gTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCCCAACTTCCGTTCCCg                                                                >  1:1428646/1‑151 (MQ=255)
                                                                                  gTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCg                                                                >  2:1745831/1‑151 (MQ=255)
                                                                                  gTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCg                                                                <  1:1737410/151‑1 (MQ=255)
                                                                                    cATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGc                                                              >  2:2308782/1‑151 (MQ=255)
                                                                                    cATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGc                                                              >  2:2308970/1‑151 (MQ=255)
                                                                                       ttAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTg                                                           <  1:538365/151‑1 (MQ=255)
                                                                                       ttAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTGCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTg                                                           <  1:1523669/151‑1 (MQ=255)
                                                                                            cAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCg                                                      >  1:2171944/1‑151 (MQ=255)
                                                                                            cAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCg                                                      <  1:940825/151‑1 (MQ=255)
                                                                                            cAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCg                                                      >  2:1682296/1‑151 (MQ=255)
                                                                                            cAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCGTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCg                                                      <  2:610124/151‑1 (MQ=255)
                                                                                             aGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCgg                                                     >  1:2436496/1‑151 (MQ=255)
                                                                                             aGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCgg                                                     >  1:2307408/1‑151 (MQ=255)
                                                                                             aGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCgg                                                     >  1:2307496/1‑151 (MQ=255)
                                                                                              ggCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCggg                                                    <  1:1170005/151‑1 (MQ=255)
                                                                                              ggCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCggg                                                    <  1:2538838/151‑1 (MQ=255)
                                                                                                cAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGCGGTGCGGCGTTCCAACGGGTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCACCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCAGCCGCGGAGCGGGaa                                                  >  2:2593748/1‑151 (MQ=255)
                                                                                                cAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGaa                                                  >  1:1858042/1‑151 (MQ=255)
                                                                                                    cTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCg                                              <  1:1812371/151‑1 (MQ=255)
                                                                                                     tGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGTGAAACCGa                                             <  1:1071699/151‑1 (MQ=255)
                                                                                                      gCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCgca                                            >  2:408649/1‑149 (MQ=255)
                                                                                                      gCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGaa                                            >  2:1827915/1‑151 (MQ=255)
                                                                                                      gCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGaa                                            <  1:32752/151‑1 (MQ=255)
                                                                                                       cATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAAc                                           <  1:1804243/151‑1 (MQ=255)
                                                                                                       cATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAAc                                           <  1:1533470/151‑1 (MQ=255)
                                                                                                          cTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCa                                        >  1:1840062/1‑151 (MQ=255)
                                                                                                            ggcaggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGc                                      >  1:482066/1‑151 (MQ=255)
                                                                                                            ggcaggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGc                                      >  2:745234/1‑151 (MQ=255)
                                                                                                              caggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGGGGGAACCCGAACGCAGCGa                                    >  2:854642/1‑151 (MQ=255)
                                                                                                              caggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGa                                    >  2:707665/1‑151 (MQ=255)
                                                                                                              caggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGa                                    >  2:1801006/1‑151 (MQ=255)
                                                                                                                ggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGCCCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGCGCGGGAAACCGAACGCAGCGAAc                                  >  2:1154853/1‑151 (MQ=255)
                                                                                                                ggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAAc                                  >  2:700783/1‑151 (MQ=255)
                                                                                                                ggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAAc                                  >  1:2298019/1‑151 (MQ=255)
                                                                                                                  caTCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTg                                <  2:422148/151‑1 (MQ=255)
                                                                                                                    tCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGa                              <  1:951031/151‑1 (MQ=255)
                                                                                                                    tCCTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTATTGGCGCTTCCGGCACAACTTCCGTTCCCGACGTTCAGCGGGAAACCGAACGCAGCGCACTGGa                              >  2:2174035/1‑151 (MQ=255)
                                                                                                                      cTGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATa                            <  2:440748/151‑1 (MQ=255)
                                                                                                                       tGACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATaa                           <  1:2456392/151‑1 (MQ=255)
                                                                                                                        gACAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAc                          <  2:1162675/151‑1 (MQ=255)
                                                                                                                         aCAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAcc                         <  2:467051/151‑1 (MQ=255)
                                                                                                                          cAAGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCa                        <  2:1458463/151‑1 (MQ=255)
                                                                                                                           aaGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAg                       <  2:2307383/151‑1 (MQ=255)
                                                                                                                           aaGGCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAg                       <  2:1756290/151‑1 (MQ=255)
                                                                                                                             ggCAAGTCCGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCt                     >  1:1628600/1‑151 (MQ=255)
                                                                                                                                    ccGCGCTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGt              <  2:2084710/151‑1 (MQ=255)
                                                                                                                                     cgcgCTGATGTGCGGCGTTCCAACGGTTGCTGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGtt             >  2:1276568/1‑151 (MQ=255)
                                                                                                                                      gcgcTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTc            >  2:1441031/1‑151 (MQ=255)
                                                                                                                                       cgcTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCa           <  1:914826/151‑1 (MQ=255)
                                                                                                                                         cTGATGTGCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACg         >  2:524354/1‑151 (MQ=255)
                                                                                                                                               tgCGGCGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTg   >  1:2598603/1‑151 (MQ=255)
                                                                                                                                               ggcggcgTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACGGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTg   <  1:1709591/150‑1 (MQ=255)
                                                                                                                                                gcggcgTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGt  >  2:1556927/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGACGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGT  >  NC_000913/2152286‑2152580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: