Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,144,6430GT32.8% 213.0 / 119.1 134E460* (GAG→TAG) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (43/47);  new base T (22/22);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 134 total aligned reads displayed.

GATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTACCGTA  >  NC_000913/2144495‑2144792
                                                                                                                                                    |                                                                                                                                                     
     aaGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTa                                                                                                                                                <  1:426466/151‑1 (MQ=255)
       gACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATg                                                                                                                                              <  1:262784/151‑1 (MQ=255)
       gACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATg                                                                                                                                              <  1:2528857/151‑1 (MQ=255)
       gACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTAACGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATg                                                                                                                                              >  1:204269/1‑151 (MQ=255)
         cATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTATCTGGGATAAGCGGATGTTCTAGCTGTATGaa                                                                                                                                            >  2:1375959/1‑151 (MQ=255)
           ttAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAAt                                                                                                                                          <  2:2090067/151‑1 (MQ=255)
           ttAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAAt                                                                                                                                          <  1:694357/151‑1 (MQ=255)
           ttAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAAt                                                                                                                                          >  1:1344165/1‑151 (MQ=255)
           ttAACCAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGGGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAAt                                                                                                                                          <  2:2586216/151‑1 (MQ=255)
             aaCGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATtc                                                                                                                                        >  2:457969/1‑151 (MQ=255)
             aaCGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtc                                                                                                                                        >  2:1168896/1‑151 (MQ=255)
             aaCGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtc                                                                                                                                        >  1:177921/1‑151 (MQ=255)
                gAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctc                                                                                                                                     <  1:1888819/151‑1 (MQ=255)
                 ggCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGCAATtcctcc                                                                                                                                    <  2:428329/150‑1 (MQ=255)
                 aGCTGAAGCGCACCGGACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGGGTTGGAGTGGAAGCCGAATATTTTTAGCTGGGATGAGCGGGTGTTCGAGCTGTATGAAACtcctcc                                                                                                                                    <  2:2123691/151‑1 (MQ=255)
                 aGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctcc                                                                                                                                    <  1:2357316/151‑1 (MQ=255)
                 aGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATtcctcc                                                                                                                                    <  1:56110/151‑1 (MQ=255)
                  gCTAAAACGCACCGAACGGGTCAATCAGGAACTGATGGCGCGCATCACTCTGGGTAACGAGGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCca                                                                                                                                   <  2:201245/151‑1 (MQ=255)
                  gCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCca                                                                                                                                   <  1:360989/151‑1 (MQ=255)
                  gCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCca                                                                                                                                   <  1:798930/151‑1 (MQ=255)
                   cTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCcac                                                                                                                                  <  1:1716602/151‑1 (MQ=255)
                               gAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGa                                                                                                                      >  2:1336723/1‑151 (MQ=255)
                                 aCAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAAc                                                                                                                    <  2:1565352/151‑1 (MQ=255)
                                       cAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGGGGTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAg                                                                                                              >  2:684269/1‑151 (MQ=255)
                                       cAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAg                                                                                                              >  1:1666215/1‑151 (MQ=255)
                                        aaTCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGGACTGGCAgg                                                                                                             <  2:1287326/151‑1 (MQ=255)
                                         aTCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgt                                                                                                            <  1:2251359/151‑1 (MQ=255)
                                         aTCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgt                                                                                                            <  1:1336741/151‑1 (MQ=255)
                                         aTCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGGGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAggg                                                                                                            >  1:1886250/1‑150 (MQ=255)
                                           cAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGgtgn                                                                                                          <  2:2173644/151‑2 (MQ=255)
                                             gCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTgg                                                                                                        <  1:751452/151‑1 (MQ=255)
                                             gCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTgg                                                                                                        <  1:133136/151‑1 (MQ=255)
                                                 cTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACg                                                                                                    <  1:1789281/151‑1 (MQ=255)
                                                   gATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAg                                                                                                  >  1:593386/1‑151 (MQ=255)
                                                   gATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAg                                                                                                  >  1:2281521/1‑151 (MQ=255)
                                                     tGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtg                                                                                                <  2:909884/151‑1 (MQ=255)
                                                     tGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtg                                                                                                >  2:651806/1‑151 (MQ=255)
                                                     tGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtg                                                                                                >  2:2640732/1‑151 (MQ=255)
                                                        aGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGGTAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgt                                                                                             >  1:205432/1‑151 (MQ=255)
                                                         gcgcATCACTCTGGCTAACGAAGCGGGCGGGATTGGCCTCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAGCTGGCAGGTGTGGTACGAgtgcgtg                                                                                            <  1:1766412/151‑1 (MQ=255)
                                                         gcgcATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgtg                                                                                            <  2:1989044/151‑1 (MQ=255)
                                                         gcgcATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgtg                                                                                            <  2:277861/151‑1 (MQ=255)
                                                           gcATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGCGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAAGTCCGCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGtgct                                                                                          >  1:1826793/1‑151 (MQ=255)
                                                            cATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGtgctg                                                                                         >  2:1709234/1‑151 (MQ=255)
                                                              tCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGcc                                                                                       <  1:631955/151‑1 (MQ=255)
                                                              tCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGcc                                                                                       <  1:2506094/151‑1 (MQ=255)
                                                               cACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCg                                                                                      <  2:1451965/151‑1 (MQ=255)
                                                                   ctGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAg                                                                                  >  1:470091/1‑151 (MQ=255)
                                                                    tGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGa                                                                                 >  2:206549/1‑151 (MQ=255)
                                                                       cTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGACTATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCg                                                                              <  2:2138918/151‑1 (MQ=255)
                                                                       cTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCg                                                                              >  1:2666841/1‑151 (MQ=255)
                                                                         aaCGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGcc                                                                            <  1:2375250/151‑1 (MQ=255)
                                                                         aaCGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGcc                                                                            >  1:462521/1‑151 (MQ=255)
                                                                           cGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAg                                                                          <  1:1029648/151‑1 (MQ=255)
                                                                            gAAGCGTGCGGGATTGGCATCCGGGCGTGGGAGTTGAAGCCGAATATTTTTAGCTGCGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGTCAGTTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCCGc                                                                         >  2:293607/1‑151 (MQ=255)
                                                                             aaGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCa                                                                        >  1:1107309/1‑151 (MQ=255)
                                                                                cgggcgggATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGc                                                                     <  1:162763/151‑1 (MQ=255)
                                                                                 gggcgggATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATAAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGcc                                                                    >  2:413915/1‑151 (MQ=255)
                                                                                        aTTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAg                                                             <  1:2620116/151‑1 (MQ=255)
                                                                                         ttGGCATCGGGGATTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGGAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgt                                                            <  1:1367513/151‑1 (MQ=255)
                                                                                             cATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGGAGATCGCCAGCATGCCGAAAAAgtgatt                                                        >  1:2262713/1‑151 (MQ=255)
                                                                                                cTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgtgattcgt                                                     >  2:2533548/1‑151 (MQ=255)
                                                                                                cTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgtgattcgt                                                     <  2:1107294/151‑1 (MQ=255)
                                                                                                cTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgtgattcgt                                                     >  2:1534762/1‑151 (MQ=255)
                                                                                                  nggagtgggagtTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgtgattcgtga                                                   >  2:2233876/2‑151 (MQ=255)
                                                                                                     agtgggagtTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAgtgattcgtgattc                                                <  1:1663467/151‑1 (MQ=255)
                                                                                                          gagtTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGACGAAAAAGTGATTCGTGATTCGTTGc                                           <  1:2008153/151‑1 (MQ=255)
                                                                                                             ttGAAGCCGAAAATTTATAGCAGGGATAAGCGGATGTTCGAGCTGTATGAAAGTCCTCCACATATCAAACCCAACTGGCAGGTGTGGTAAGAGTGCGTGCTGCCTGAAGATCAAAAAAAAAAAAAAAAAGTGATTCGTGATTCGTTGCAAt                                        <  2:1635215/151‑1 (MQ=255)
                                                                                                              tGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGGGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATc                                       >  1:194168/1‑151 (MQ=255)
                                                                                                              tGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGCTACGAGAGCGTTCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTTGTGATTCGTTGCGAtt                                       >  2:393197/1‑150 (MQ=255)
                                                                                                                aaGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCAc                                     >  2:1838284/1‑151 (MQ=255)
                                                                                                                 aGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACg                                    >  2:1216372/1‑151 (MQ=255)
                                                                                                                    cGAATATTTTTAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTc                                 <  1:493569/151‑1 (MQ=255)
                                                                                                                    cGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTc                                 >  1:1993913/1‑151 (MQ=255)
                                                                                                                    cGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTc                                 >  1:2397500/1‑151 (MQ=255)
                                                                                                                    cGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTc                                 >  1:1242434/1‑151 (MQ=255)
                                                                                                                    cGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACAGCACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTc                                 >  1:844816/1‑151 (MQ=255)
                                                                                                                     gAATATTTTTAGCTGGGATAAGCGGATGTTCTAGATGCATGAATTTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCg                                >  2:1835663/1‑151 (MQ=255)
                                                                                                                     gAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGATTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCg                                >  2:2026566/1‑151 (MQ=255)
                                                                                                                          tttttAGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCtt                           <  1:1444853/151‑1 (MQ=255)
                                                                                                                          tttttAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCtt                           >  1:1889197/1‑151 (MQ=255)
                                                                                                                            tttAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTa                         <  1:66749/151‑1 (MQ=255)
                                                                                                                               aGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGCACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTacac                      >  2:632560/1‑151 (MQ=255)
                                                                                                                               aGCTGGGATAAGCGGATGTTCTAGCTGTATGAAATTCCTCAACATATCAAACCGAACTGGAAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAAc                      <  1:293612/151‑1 (MQ=255)
                                                                                                                                 cTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTg                    >  2:2066629/1‑151 (MQ=255)
                                                                                                                                  tGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCATATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTgg                   <  1:686420/151‑1 (MQ=255)
                                                                                                                                  tGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTgg                   <  1:895102/151‑1 (MQ=255)
                                                                                                                                   gggATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGa                  <  1:2082691/151‑1 (MQ=255)
                                                                                                                                    ggATAAGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGaa                 <  1:1420710/151‑1 (MQ=255)
                                                                                                                                         aGCGGATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCg            >  2:1089394/1‑151 (MQ=255)
                                                                                                                                         aGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCg            >  2:2010725/1‑151 (MQ=255)
                                                                                                                                            ngATGTTCTAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCAt         >  2:1239915/2‑151 (MQ=255)
                                                                                                                                            ggATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCAAAAAAAAAAAAAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCAt         <  2:177918/151‑1 (MQ=255)
                                                                                                                                               tGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAc      >  2:1619058/1‑151 (MQ=255)
                                                                                                                                               tGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAc      <  1:1709256/151‑1 (MQ=255)
                                                                                                                                                gTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAcc     <  1:2595933/151‑1 (MQ=255)
                                                                                                                                                gTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAcc     <  2:771649/151‑1 (MQ=255)
                                                                                                                                                  tCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCACTCACGCTCGCCCTTTACACTGGAATTTCGCATTACCGt   >  2:1391821/1‑151 (MQ=255)
                                                                                                                                                  tCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTACCGt   >  2:2171205/1‑151 (MQ=255)
                                                                                                                                                   cGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTACCGTa  >  1:654793/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTACCGTA  >  NC_000913/2144495‑2144792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑