Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,458,110 C→A T578T (ACC→ACA)  gspD → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,1100CA100.0% 561.3 / NA 156T578T (ACC→ACA) gspDgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (81/75);  total (81/75)

Only 100 of 159 total aligned reads displayed.

TACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457963‑3458256
                                                                                                                                                   |                                                                                                                                                  
tACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                                 >  2:1861377/1‑151 (MQ=255)
tACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                                 >  2:2589495/1‑151 (MQ=255)
 aCTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                <  1:1886041/151‑1 (MQ=255)
    aTTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                             >  1:1393941/1‑151 (MQ=255)
       cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCCCAGAGCGCGCTa                                                                                                                                          >  1:1008340/1‑151 (MQ=255)
       cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTa                                                                                                                                          >  1:528556/1‑151 (MQ=255)
        aaaaCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTaa                                                                                                                                         >  1:1983976/1‑151 (MQ=255)
           aCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACg                                                                                                                                      <  1:1025984/151‑1 (MQ=255)
                gTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAcc                                                                                                                                 >  1:2004881/1‑151 (MQ=255)
                gTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAcc                                                                                                                                 <  2:814108/151‑1 (MQ=255)
                 tGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCt                                                                                                                                <  2:1669050/151‑1 (MQ=255)
                   cTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                              >  2:2119006/1‑151 (MQ=255)
                    tGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAt                                                                                                                             >  1:1008322/1‑151 (MQ=255)
                     ggTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGCGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                            >  1:473466/1‑151 (MQ=255)
                          aaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGGGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                       <  2:2348333/151‑1 (MQ=255)
                          aaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                       >  1:1975591/1‑151 (MQ=255)
                            aCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTa                                                                                                                     <  1:68895/151‑1 (MQ=255)
                                 tGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgt                                                                                                                >  1:1860814/1‑151 (MQ=255)
                                  gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgtc                                                                                                               <  1:548946/151‑1 (MQ=255)
                                        gtggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcgacga                                                                                                         <  1:1099268/151‑1 (MQ=255)
                                         tggtCCTTGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAt                                                                                                        <  1:827953/151‑1 (MQ=255)
                                          ggtCCTGGGCGGATTGCTGGATGATTTTTTTTTTTTTTTTTTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGCCGAtt                                                                                                       >  2:2666886/1‑151 (MQ=255)
                                             ccTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATc                                                                                                    <  1:216077/151‑1 (MQ=255)
                                              cTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTCAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  2:1220712/1‑151 (MQ=255)
                                              cTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  1:1455868/1‑151 (MQ=255)
                                               tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCg                                                                                                  >  2:940914/1‑151 (MQ=255)
                                                 ggCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTg                                                                                                <  1:2609736/151‑1 (MQ=255)
                                                         gCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatgat                                                                                        >  2:152199/1‑151 (MQ=255)
                                                          cTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGatgatg                                                                                       <  1:742021/151‑1 (MQ=255)
                                                           tGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGt                                                                                      >  1:2063655/1‑151 (MQ=255)
                                                            ggATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                     >  1:1728330/1‑151 (MQ=255)
                                                            ggATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                     <  1:1053192/151‑1 (MQ=255)
                                                               tgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAt                                                                                  <  1:112171/151‑1 (MQ=255)
                                                               tgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAt                                                                                  <  2:384224/151‑1 (MQ=255)
                                                                gatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCGGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATc                                                                                 <  2:1616225/151‑1 (MQ=255)
                                                                 atTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCg                                                                                <  1:2541649/151‑1 (MQ=255)
                                                                  tttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGc                                                                               >  1:2049248/1‑151 (MQ=255)
                                                                    tttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGTTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTc                                                                             <  1:1199121/151‑1 (MQ=255)
                                                                      tCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGATAAACGCAAACTGATGGTATTTATTCGTCTGAAGATTATTCGTGACGATGATGTTTATCGCTCAc                                                                           >  1:1920394/1‑151 (MQ=255)
                                                                       cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACt                                                                          >  1:968413/1‑151 (MQ=255)
                                                                        gaaaGGGCAAGTGTCAAAGGTTCCTCTGCTTTGCATTAGTCGTTTAGTGGGGCAATTCTTCCGCTATACCTACTCAGAGAGCGCTAGACGCAACCTGATGGTATTTACCCGTCCGACTATTATCCGTGATGATGATGTTTATCGCTCACTg                                                                         <  2:70619/150‑1 (MQ=255)
                                                                           agagCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCa                                                                      <  1:678884/151‑1 (MQ=255)
                                                                               cAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAag                                                                  <  2:2431302/151‑1 (MQ=255)
                                                                               cAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAag                                                                  <  1:454389/151‑1 (MQ=255)
                                                                               cAAGAGTCAACGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAag                                                                  <  1:599368/151‑1 (MQ=255)
                                                                                 aGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGCTGATGTTTATCGCTCACTGTCAAAagag                                                                >  2:543866/1‑151 (MQ=255)
                                                                                  gtgtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAgaga                                                               <  2:2342163/151‑1 (MQ=255)
                                                                                    gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                             <  2:968399/151‑1 (MQ=255)
                                                                                    gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                             <  2:2428281/151‑1 (MQ=255)
                                                                                    gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                             <  2:2580038/151‑1 (MQ=255)
                                                                                    gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                             <  1:2444827/151‑1 (MQ=255)
                                                                                     tCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAAt                                                            <  2:914347/151‑1 (MQ=255)
                                                                                      cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                           <  1:2358194/151‑1 (MQ=255)
                                                                                      cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                           >  1:2170903/1‑151 (MQ=255)
                                                                                       aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATac                                                          >  1:1701365/1‑151 (MQ=255)
                                                                                       aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTAACTGTCAAAAGAGAAATac                                                          <  2:854816/151‑1 (MQ=255)
                                                                                         aGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATacac                                                        >  2:1283161/1‑151 (MQ=255)
                                                                                          ggTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACAcc                                                       <  1:2350697/151‑1 (MQ=255)
                                                                                          ggTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACAcc                                                       <  1:2554318/151‑1 (MQ=255)
                                                                                            ttCCTCTGCTTGGCGATATTCCTTTAGTGGGGCCGCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  2:2541435/151‑1 (MQ=255)
                                                                                            ttCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  1:1093746/151‑1 (MQ=255)
                                                                                            ttCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  1:122910/151‑1 (MQ=255)
                                                                                            ttCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCCCCACAGAGCGCGCTAAACGCAACATGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  1:989579/151‑1 (MQ=255)
                                                                                               ctctGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTa                                                  >  1:2619815/1‑151 (MQ=255)
                                                                                                   gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGt                                              >  1:2491690/1‑151 (MQ=255)
                                                                                                    nttGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTc                                             >  2:2507341/2‑151 (MQ=255)
                                                                                                    cTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTc                                             >  1:89674/1‑151 (MQ=255)
                                                                                                      tGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAg                                           <  1:1493279/151‑1 (MQ=255)
                                                                                                        gCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         >  1:1946617/1‑151 (MQ=255)
                                                                                                         cGATATTCCTTTAGTGGCGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGCTGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGac                                        >  2:1883796/1‑150 (MQ=255)
                                                                                                             atTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCGCTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaac                                    <  2:2491653/151‑1 (MQ=255)
                                                                                                              ttCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGCTATACACCCGTTACCGTCAGGAGcaaca                                   <  2:687100/151‑1 (MQ=255)
                                                                                                              ttCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaaca                                   >  2:135226/1‑151 (MQ=255)
                                                                                                                 cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATCCACCCGTTACCGTCAGGAGCAacaaca                                >  2:881996/1‑151 (MQ=255)
                                                                                                                     aGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCgg                            >  1:144152/1‑151 (MQ=255)
                                                                                                                      gTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGCTTATCCGTGACGATGATGTTTATCGCTCACTGCCACAAGAGAAACACACCCGTTCCCGTCAGGAGCAACAACAGCGGa                           >  2:1710777/1‑151 (MQ=255)
                                                                                                                      gTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                           >  2:300236/1‑151 (MQ=255)
                                                                                                                      gTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                           >  1:1791254/1‑151 (MQ=255)
                                                                                                                        ggggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAA‑ACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        >  1:481536/1‑151 (MQ=255)
                                                                                                                          ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCGAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCgc                       >  2:566263/1‑150 (MQ=255)
                                                                                                                          ggCAACTCTTCCGCTATACCTCCAAAGAGCGCGCTAAACGCAACCTGATGGTATTTATCGGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTAAAAAGAGAAATACACGCGTTACAGTCAGGAGCAAAAAAAGCGGATCGa                       >  1:390022/1‑151 (MQ=255)
                                                                                                                           gCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCATGAGCAACAACAGCgttgacaa                      >  2:2104512/1‑144 (MQ=255)
                                                                                                                            cAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACg                     <  1:2423897/151‑1 (MQ=255)
                                                                                                                               ctctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGa                  <  2:1200598/151‑1 (MQ=255)
                                                                                                                               ctctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGa                  >  1:1257979/1‑151 (MQ=255)
                                                                                                                                 ctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATGCACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaaa                >  2:1264416/1‑151 (MQ=255)
                                                                                                                                 ctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaaa                >  2:1100541/1‑151 (MQ=255)
                                                                                                                                 ctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaaa                >  1:1207103/1‑151 (MQ=255)
                                                                                                                                  ttCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAAt               <  2:1130762/151‑1 (MQ=255)
                                                                                                                                        tataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg         <  1:1636451/151‑1 (MQ=255)
                                                                                                                                         ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCAGTCCGGCGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgc        >  1:861340/1‑151 (MQ=255)
                                                                                                                                          taCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcg       >  1:747014/1‑151 (MQ=255)
                                                                                                                                            ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  2:2222393/1‑151 (MQ=255)
                                                                                                                                            ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  2:1645242/1‑151 (MQ=255)
                                                                                                                                            ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  2:160308/1‑151 (MQ=255)
                                                                                                                                            ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  1:1594519/1‑151 (MQ=255)
                                                                                                                                            ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  1:1865616/1‑151 (MQ=255)
                                                                                                                                             cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTg    <  1:1789825/151‑1 (MQ=255)
                                                                                                                                               ccACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  <  1:29791/151‑1 (MQ=255)
                                                                                                                                               ccACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTAAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  >  1:2231774/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457963‑3458256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑