Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,419,549 +C intergenic (‑38/+98) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,419,5481.C93.2% 139.8 / 3.8 44intergenic (‑37/+99)PP_1242/PP_1244hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (22/19);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAT  >  NC_002947/1419410‑1419678
                                                                                                                                            |                                                                                                                                   
gATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                    >  1:327607/1‑140 (MQ=255)
gATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                    <  2:327607/140‑1 (MQ=255)
 aTGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCgg                                                                                                                          <  7:80910/149‑1 (MQ=255)
     cGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCAc                                                                                                                      >  4:283125/1‑149 (MQ=255)
               gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTccg                                                                                                            >  1:133150/1‑148 (MQ=255)
               gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTcc                                                                                                             >  3:151258/1‑148 (MQ=255)
                cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCca                                                                                                           >  2:310405/1‑149 (MQ=255)
                 ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCcac                                                                                                          <  3:82069/149‑1 (MQ=255)
                  gAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCcaca                                                                                                         <  3:283125/149‑1 (MQ=255)
                    aCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGGAGTGTGGTGGGTTCTGTTGTGTTTTCGGTGGGAATTTTTTTGTTTCCCTGGATTCTGGGTTGCATGGGATGG‑CATATTCGCGGGCACGCCCGCTCCCCCAg                                                                                                        >  1:270543/1‑149 (MQ=255)
                        cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                   >  2:52902/1‑149 (MQ=255)
                           gggAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGGCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                <  4:280345/147‑1 (MQ=255)
                           gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                <  1:310405/149‑1 (MQ=255)
                            tGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGc                                                                                               <  2:133150/149‑1 (MQ=255)
                              aGTTTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCa                                                                                              <  6:29212/148‑1 (MQ=255)
                                tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTg                                                                                           >  5:194039/1‑149 (MQ=255)
                                  ttgttTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCg                                                                                         >  3:220560/1‑149 (MQ=255)
                                     ttttCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCAGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCTCCGGCACGCCCGCTCCCACATGTACAGTGCATTCCGTGa                                                                                      >  2:287007/1‑149 (MQ=255)
                                          gCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                        >  7:310733/1‑126 (MQ=255)
                                           ccTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtg                                                                                >  4:102713/1‑149 (MQ=255)
                                             ttGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                              >  6:88704/1‑149 (MQ=255)
                                               ggTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg                                                                             <  4:220560/148‑1 (MQ=255)
                                                gttgcaGCCGGTCGTGCC‑GCCGGTGGTGGGTGCGGTTGTGGTGTCGGGTGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtggt                                                                            <  6:263101/143‑1 (MQ=37)
                                                   gAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAcc                                                                        <  4:151258/149‑1 (MQ=255)
                                                    aaGCAGGTCTTGCC‑GAGTGTGGTGGGTTCTGTTGTGTTGTCGGTGGTCTTTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CGTCTTCGCGGGCAAGCCCGGTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAcc                                                                        <  1:287007/148‑1 (MQ=255)
                                                           tCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTccc                                                                                                            >  7:110758/1‑105 (MQ=255)
                                                             ttGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTACCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACACCCGCTCCCACAGGGACTGTGCTGTGCGTGACGGCTGTGGTGACCCTATGGGAGc                                                              >  4:71858/1‑149 (MQ=255)
                                                                 c‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTACCACAGGTACGGGGCAGGGCGTGACGGGTGTGGTGCCCCGGGTGGAGCCGGc                                                          >  4:330771/1‑149 (MQ=255)
                                                                         ggtggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcg                                                   >  5:68802/1‑149 (MQ=255)
                                                                            gggTTCTGTTGTGTTGTCGGGGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGa                                                <  1:52902/149‑1 (MQ=255)
                                                                               ttCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAaga                                             <  3:102713/149‑1 (MQ=255)
                                                                                 cTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAagag                                            <  6:194039/148‑1 (MQ=255)
                                                                                   gttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                          >  1:138351/1‑132 (MQ=255)
                                                                                   gttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                          <  2:138351/132‑1 (MQ=255)
                                                                                      gtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    >  4:109033/1‑119 (MQ=255)
                                                                                      gtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgt                                                                    <  3:109033/119‑1 (MQ=255)
                                                                                                     aTTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCCCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCGAATGCAAgg                       >  3:344781/1‑149 (MQ=255)
                                                                                                         ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                              <  4:290018/90‑1 (MQ=255)
                                                                                                         ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                              >  3:290018/1‑90 (MQ=255)
                                                                                                         ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTa                               >  1:69295/1‑137 (MQ=255)
                                                                                                                  ccTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGtgtg                   <  2:50778/140‑1 (MQ=255)
                                                                                                                  ccTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGtgtg                   >  1:50778/1‑140 (MQ=255)
                                                                                                                      gATTCTGGGTTGCCTGGGCGGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCTGGGCGTTAGGGCTGTGGTGACCCTGTGGTTGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTggg       <  2:270543/148‑1 (MQ=37)
                                                                                                                      gATTCGGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACCGGTACTGGGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGCGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTgg        <  7:198496/147‑1 (MQ=37)
                                                                                                                        ttCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGGGACGGCTGTGGTGACCCAGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGGTGGTa     >  2:227428/1‑148 (MQ=37)
                                                                                                                           tGGGTTGCGTGGGCGGG‑CGTCTTCGCGGCCACGCCCGCTCCCACTGGTCCTGTGCTGTGCGTGCCGGGGGTGGTGCCCGTGGGGGTGGGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAt  <  4:344781/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                   
GATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCC‑GACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAAT  >  NC_002947/1419410‑1419678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑