Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4670TG26.8% 39.0 / 24.5 41intergenic (+32/+39)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (13/17);  new base G (7/4);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGC  >  NC_002947/5740320‑5740610
                                                                                                                                                   |                                                                                                                                                
gCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGt                                                                                                                                                  <  5:79058/148‑1 (MQ=255)
   aGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCaa                                                                                                                                              <  3:164539/149‑1 (MQ=255)
               tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAgg                                                                                                                                   <  7:219188/148‑1 (MQ=255)
                gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATAGCGTGCAAGCCCGCGTACGGt                                                                                                                                 >  1:213523/1‑149 (MQ=255)
                 aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCACCCCATCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                >  7:122582/1‑149 (MQ=255)
                      gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaa‑c                                                                                                                          >  2:223257/1‑149 (MQ=255)
                      gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGcttgc                                                                                                                                            >  6:5453/1‑128 (MQ=255)
                      gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGcttgc                                                                                                                                            <  5:5453/132‑5 (MQ=255)
                      gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTg                                                                                                                               >  6:266742/1‑145 (MQ=38)
                      gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTg                                                                                                                               <  5:266742/145‑1 (MQ=38)
                       aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGACAGCGGGCGGGCGTTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaa‑cg                                                                                                                         <  7:361673/149‑1 (MQ=255)
                              aaTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaa‑c                                                                                                                          <  1:59364/141‑1 (MQ=38)
                              aaTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaa‑c                                                                                                                          >  2:59364/1‑141 (MQ=38)
                                                    gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACCGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                             <  1:223257/149‑1 (MQ=255)
                                                           cAGGAAGATGACCGCGGGCGGGCTTTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGc                                                                                     <  2:213523/149‑1 (MQ=255)
                                                               aaGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGAt                                                                                 <  5:110433/149‑1 (MQ=255)
                                                                    gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                <  6:289084/98‑1 (MQ=255)
                                                                    gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                >  5:289084/1‑98 (MQ=255)
                                                                         cgggcgggCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGCGCCCATAGCGGGGAAGCCCCCGCCGGGTTGGCC‑CGCAACCCCCAAAAACGCAGGGGGTTTTTTAGATTTTCTGAGGGGGcgcccg                                                                       >  2:37127/1‑149 (MQ=255)
                                                                           ggcgggctTTTGACAAGGTGTTGCGCAAGATTTTGGGCTGAGCGTACCGGGTTTGCTTTGAAGCCCTTCGCGTTCAATCCCGCTAAGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgcc                                                                     <  5:205070/149‑1 (MQ=255)
                                                                                          aGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTGGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTgg                                                      <  1:37127/149‑1 (MQ=255)
                                                                                              gtTGCGCCAGCTTTGGGGCGGAGCGTACCGGGGTTCCGTTGCACCCCTTCGCGTGCAAGCCCGCTAAGGGTTGAAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc                                                  <  7:153156/149‑1 (MQ=255)
                                                                                                  cgcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                              <  5:356833/149‑1 (MQ=255)
                                                                                                         ttttGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAccc                                            <  7:184191/144‑1 (MQ=255)
                                                                                                             gggCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                    <  5:121478/148‑1 (MQ=255)
                                                                                                               gCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGCGTTCAAGCCCGCCAACGGTTTCAA‑CGCCACCCCAAAAAAAAAAGTGCAATTTTAATATTTTCTGATCGGACCCCCGCCACATAAAACCGCTGGTGTAAAAACCCAGCTGCcccg                                 >  2:55647/1‑149 (MQ=255)
                                                                                                                                  ggtttcAGACCTTCGCGGGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtct               <  6:377684/147‑1 (MQ=255)
                                                                                                                                  cgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACACCTGCTGCCGCGCCCTGGACAGCAGGTtct               >  1:83767/1‑148 (MQ=255)
                                                                                                                                   gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                           <  4:149154/119‑1 (MQ=255)
                                                                                                                                   gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                           >  3:149154/1‑119 (MQ=255)
                                                                                                                                       cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                             <  4:134323/129‑1 (MQ=255)
                                                                                                                                       cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                             >  3:134323/1‑129 (MQ=255)
                                                                                                                                        aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc                                                  >  2:314774/1‑107 (MQ=255)
                                                                                                                                        aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc        >  5:52206/1‑149 (MQ=255)
                                                                                                                                         gCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc       <  6:52206/149‑1 (MQ=255)
                                                                                                                                            cTTCGCGGGCTTGCACGCGAAGGGCTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg    >  5:58087/1‑149 (MQ=39)
                                                                                                                                            cTTCGCGGGCTTGCACGCGAAGGGCTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg    >  6:183469/1‑149 (MQ=39)
                                                                                                                                             ttCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg   >  4:311551/1‑149 (MQ=255)
                                                                                                                                             ttCGCGGGCTTGCACGCGAAGGGCTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg   >  5:203087/1‑149 (MQ=39)
                                                                                                                                              tCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc  >  7:42311/1‑149 (MQ=255)
                                                                                                                                                gcgTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                <  5:22626/133‑1 (MQ=255)
                                                                                                                                                gcgTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCAAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                >  6:22626/1‑133 (MQ=255)
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GCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAA‑CGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGC  >  NC_002947/5740320‑5740610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑