Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 353,067 A→G intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG98.0% 165.6 / NA 50intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (24/25);  minor base T (1/0);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACC  >  NC_002947/352921‑353211
                                                                                                                                                  |                                                                                                                                                  
ttCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGAACGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCggg                                                                                                                                                  >  2:100948/1‑149 (MQ=255)
 tCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCggg                                                                                                                                                  >  1:345776/1‑148 (MQ=255)
     gAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑c                                                                                                                                              <  6:123306/147‑1 (MQ=255)
         ggCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCG‑TGCCCGc                                                                                                                                        <  1:271919/149‑1 (MQ=255)
          gCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑Cccccccg                                                                                                                                       >  6:44323/1‑146 (MQ=255)
           cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGccc                                                                                                                                          <  4:370871/145‑1 (MQ=255)
           cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGccc                                                                                                                                          >  3:370871/1‑145 (MQ=255)
            aTGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTcc                                                                                                                                     <  3:99279/149‑1 (MQ=255)
                        tGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCCCCCCAAAGGTTTgg                                                                                                                          >  5:246422/1‑148 (MQ=255)
                          cTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGttt                                                                                                                            >  6:111509/1‑144 (MQ=255)
                          cTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGttt                                                                                                                            <  5:111509/144‑1 (MQ=255)
                             gcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGAt                                                                                                                    <  2:345776/149‑1 (MQ=255)
                                  cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCtg                                                                                                               >  4:221770/1‑149 (MQ=255)
                                  cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCACCCACAGGGTTGCTGAGAACTAtg                                                                                                               >  4:30791/1‑149 (MQ=39)
                                      aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTggg                                                                                                           <  3:39657/149‑1 (MQ=255)
                                          tCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCCCCGGTTTGCTGAGCTGGGTGTGGG‑AGCg                                                                                                      >  6:297813/1‑149 (MQ=38)
                                           ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCgg                                                                                                     >  4:266585/1‑149 (MQ=255)
                                            cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCggg                                                                                                    >  6:104618/1‑149 (MQ=255)
                                            cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCggg                                                                                                    >  4:268003/1‑149 (MQ=255)
                                            cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCgg                                                                                                     <  7:284688/148‑1 (MQ=255)
                                                    cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCcg                                                                                            >  7:250051/1‑149 (MQ=255)
                                                     gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCcgc                                                                                           >  5:152565/1‑149 (MQ=255)
                                                          cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAgg                                                                                      >  3:328859/1‑149 (MQ=255)
                                                           acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGt                                                                                                 <  1:78859/137‑1 (MQ=255)
                                                           acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGt                                                                                                 >  2:78859/1‑137 (MQ=255)
                                                           acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGAACGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACCGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAggg                                                                                     <  1:100948/149‑1 (MQ=255)
                                                               tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTGTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCcgc                                                                                 <  1:225246/149‑1 (MQ=255)
                                                                catgtaGGCCTTGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTgg                                                                                                            <  5:88871/117‑1 (MQ=37)
                                                                catgtaGGCCTTGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTgg                                                                                                            >  6:88871/6‑122 (MQ=37)
                                                                 ccGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCcgcg                                                                                >  1:219698/1‑148 (MQ=255)
                                                                       gCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGc                                                                         <  2:219698/149‑1 (MQ=255)
                                                                       gCCAAGGCCTACATGGCTTCGCGCGGGATCGGCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGc                                                                         <  3:221770/149‑1 (MQ=255)
                                                                             gCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCGTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGAGCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAAt                                                                   <  5:117865/149‑1 (MQ=255)
                                                                               cTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTg                                                                 <  5:104618/149‑1 (MQ=255)
                                                                                 aCATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCAGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCt                                                               >  1:271801/1‑149 (MQ=255)
                                                                                     ggCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGGTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCaa                                                           >  7:177971/1‑149 (MQ=255)
                                                                                       cTTCGCGCGGGATCGGCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTGCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAAcc                                                         <  5:297813/149‑1 (MQ=255)
                                                                                           gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTg                                                                 >  4:266367/1‑137 (MQ=255)
                                                                                           gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTg                                                                 <  3:266367/137‑1 (MQ=255)
                                                                                                ggATCGTCGTTCGCTGATCCTTTGACCGGGGCTGCTTTGCAGCGCTGCGCGGTCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTTGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGTCCCCAATTTCTTCAACCGTGGTTCtt                                                <  7:289373/149‑1 (MQ=38)
                                                                                                 gATCGTCGTTCGCTGATCCATTGACCGGGGGTGCTTTGCAGCCCTTCGCTGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTg                                               >  1:30403/1‑149 (MQ=255)
                                                                                                     gtcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGcc                                             >  3:343586/1‑147 (MQ=255)
                                                                                                     ggcgtTCGCTGAGCCATTGACCGGGGCTGCTTGGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGa                                           <  4:343586/147‑1 (MQ=255)
                                                                                                      tcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCcgc                                                                                           >  1:11885/1‑100 (MQ=255)
                                                                                                      tcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCcgc                                                                                           <  2:11885/100‑1 (MQ=255)
                                                                                                      tcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                          <  6:152565/149‑1 (MQ=255)
                                                                                                           cGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcagc                                      <  3:268003/148‑1 (MQ=255)
                                                                                                                  ccATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGt                               >  3:227021/1‑148 (MQ=255)
                                                                                                                        cccGGGGGTGCTTGGATGCCCTTCGGGGGCA‑CGCCGGCTCCACCCGGTTGGCTGTCATGTC‑GTGGGTTGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                                    <  7:354157/136‑1 (MQ=19)
                                                                                                                         ccGGGGCTGCTTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGCGGTGTCTGTGGG‑AGCGGGCGTGCCCGCGACGGGCCGCGCAGCGGCCCCAATTGCTTCCACCGGGGGTCGTGGCGGGCAGCGCGTGGGTAAGCTCg                       >  7:340501/1‑149 (MQ=25)
                                                                                                                                tgctTTGCAGCCCTTCGCGGGCA‑CGCCCGCTCCCCCAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATccc                >  5:340935/1‑149 (MQ=255)
                                                                                                                                         gCCCTTCGCGGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATcc       <  7:280688/149‑1 (MQ=255)
                                                                                                                                               cgcgGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCccacc  <  4:328859/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCA‑CGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGG‑AGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACC  >  NC_002947/352921‑353211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑