| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,740,819 | 2 bp→CT | intergenic (+56/+696) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,819 | 0 | T | C | 91.4% | 90.5 / 1.2 | 37 | intergenic (+56/+697) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (20/12); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01 | |||||||||||
| * | NC_002947 | 4,740,820 | 0 | G | T | 91.7% | 107.0 / 4.3 | 37 | intergenic (+57/+696) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base T (20/13); minor base . (2/1); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740679‑4740954 || cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg < 6:39262/147‑1 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCgg > 2:300478/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCgg < 3:102699/149‑1 (MQ=255) cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTg < 2:190790/149‑1 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCcgc > 2:134704/1‑149 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCgg > 6:146092/1‑137 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCgg < 5:146092/137‑1 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGGCAAGGCCGCGAag < 3:221977/149‑1 (MQ=255) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAACGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCc‑tct < 3:51457/124‑1 (MQ=255) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTTTCT‑GTGCAGGCc‑tct > 4:46428/1‑124 (MQ=255) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCc‑tct > 4:51457/1‑124 (MQ=255) tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGTAGAGGCCGGca > 3:224332/1‑148 (MQ=255) tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca > 7:299820/1‑148 (MQ=255) ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa > 1:21436/1‑148 (MQ=255) gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcacca > 5:292363/1‑148 (MQ=255) cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑ACTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa > 3:165354/1‑148 (MQ=255) aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc > 1:137928/1‑148 (MQ=255) gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc < 2:21436/149‑1 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGaa < 3:347922/105‑1 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGaa > 4:347922/1‑105 (MQ=255) gACCGCAAGTAAGCTGCTGTAGCCTAACGCAACATGGCTGCCCTCGGCCTG‑CTTTTTTTATTTGTCGTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCTAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 2:137928/149‑1 (MQ=255) aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCATCGCGGCCGTGCCCGCGAAGCGGGCGGCGCCGCCCCCACAAAACCGCAGGTTTTGTGCGCCCCCGCACGc > 1:171735/1‑149 (MQ=255) cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTGGCGGCCTTGCCCGCGAAGAGGCCGGCACCGCCACCACAAAACCTCAGTTTTTCTCCGCCCCCTCACGccc > 4:143038/1‑147 (MQ=255) cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCGTCGCGGCCTTTCCCGCGAAGAGGCCCGCCCACACACCACAAAACCACACTTTTTCTCCCCCCGCGCACCCAg > 3:166077/1‑149 (MQ=255) cAAGTAAGCTGCAGTAGCCTAACCCATTAAGTTTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc < 1:306168/119‑1 (MQ=25) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑ACTTAGCGGCCGTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc > 2:306168/1‑119 (MQ=255) aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGc < 7:178143/74‑1 (MQ=37) aaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTAGCGGCCGTGCCCGCGAAGAGGCCGGCCCAGACACCCCAAAAACGCAGTGTTTATACCCCCCCGCACGCAGCCCATTAAGGGTATTTAACCGCGCATc > 4:180758/4‑149 (MQ=255) agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac > 2:83440/1‑148 (MQ=255) gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac > 3:62690/1‑149 (MQ=255) cTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGACTTGCCCGCGAAGAGGCCGGCCCAGCCACCACAAAACCTCAGATTTTATCCGCACGCTCACGCAGCCCTTTCAGGGTATTGACCTGGGCAACcaccac > 5:295549/1‑148 (MQ=255) tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGa > 5:91733/1‑149 (MQ=255) gggCAG‑CTTTTTTTATTTGTCGTGGGCCGCCC‑TCTTCGCGGCCTGGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg < 1:134704/147‑1 (MQ=255) gggagccttttttATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg < 7:249252/142‑1 (MQ=255) gcaagcttttttttttTGCCGTGGCCGGCCGTCTGCGGGGCGTGCCCGGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg < 4:166077/143‑1 (MQ=255) ccttttttATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac > 6:350429/3‑149 (MQ=255) ttttATTTGTCCTGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca < 5:350429/147‑1 (MQ=255) || CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCC‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740679‑4740954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |