Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8050T.92.1% 138.9 / 8.9 40intergenic (+42/+711)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base . (15/20);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740660‑4740946
                                                                                                                                                    |                                                                                                                                             
aCTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCtttt                                                                                                                                            >  5:79001/1‑149 (MQ=255)
 cTGGAAAGAAATGCTCTCAAGCCCGTACAATATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑AAAGGCTGCCCGAGGGCAGCcttctt                                                                                                                                           <  6:384515/149‑1 (MQ=255)
   ggAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCtttttta                                                                                                                                         >  5:363122/1‑148 (MQ=255)
    gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                        <  4:250617/149‑3 (MQ=255)
    gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGACCTCGGGCAGCCttttttat                                                                                                                                        >  7:397986/1‑147 (MQ=255)
    gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGGAGCCGCACGCAA‑GAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                        >  8:290469/1‑147 (MQ=255)
    gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCACTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                        >  3:407382/1‑147 (MQ=255)
    gaaagaaaTGCTCTCCAGCCCGTAAAAGATCCGCCGTCCGCGCCAGCTGTACACCGGCGAGCACAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTCCCCTCGGCCAGCCtattttat                                                                                                                                        >  2:124028/1‑147 (MQ=255)
        gaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AACTAAGCTGCAGTAGCCGAACGCAA‑AAAGGCTGCCCGAGGGCAGCcttcttgcgttcg                                                                                                                                    <  7:52857/149‑8 (MQ=39)
          aaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                   <  1:195030/148‑8 (MQ=255)
                    aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAACGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                        <  1:311497/149‑1 (MQ=255)
                    aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                        >  2:94572/1‑148 (MQ=255)
                        cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                   <  7:321529/149‑1 (MQ=255)
                          tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                 >  3:232103/1‑149 (MQ=255)
                            cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                               <  2:220560/149‑1 (MQ=255)
                             aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                              <  7:104776/149‑1 (MQ=255)
                              aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                             <  2:300498/149‑1 (MQ=255)
                               gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                            <  1:171619/149‑1 (MQ=255)
                               gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGcc                                                                                                            >  7:376278/1‑149 (MQ=255)
                                 tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                          <  7:126396/149‑1 (MQ=255)
                                   ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgggcaagg                                                                                                        <  6:90254/149‑7 (MQ=255)
                                   ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                        >  5:373637/1‑149 (MQ=255)
                                      cGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAGAAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                       >  1:344558/1‑149 (MQ=255)
                                          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                 <  5:311485/149‑1 (MQ=255)
                                             gcCAGCTGAACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                              <  3:52079/149‑1 (MQ=38)
                                                aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                           >  5:358758/1‑149 (MQ=255)
                                                aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                           <  4:232103/149‑1 (MQ=255)
                                                          cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAC‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAggac                                                                                 <  7:290469/149‑4 (MQ=37)
                                                              gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCtt                                                                                                                                              >  1:30424/1‑85 (MQ=255)
                                                              gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCtt                                                                                                                                              <  2:30424/85‑1 (MQ=255)
                                                                   gAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                        >  5:233001/1‑149 (MQ=255)
                                                                    aaGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTGGCGGGCTTGCCCGGGAAGAGGCCGGCGCAGCCACCACAAAAcc                                                                       >  6:415226/1‑149 (MQ=255)
                                                                                gTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                          >  5:274404/1‑86 (MQ=255)
                                                                                gTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                          <  6:274404/86‑1 (MQ=255)
                                                                                ctatctcTGAAAGACCGC‑‑AAGTAAGCTGCATTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCTGCATCTTCTCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                           <  7:116378/143‑1 (MQ=255)
                                                                                    cccTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                       >  7:298484/1‑149 (MQ=255)
                                                                                     ccTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATCCCGGCCTTGCCCGCGAACAGGCCCGCCCACACCCCCCAAAACCTCAGTATATCTacgccc                                                      >  6:4048/1‑149 (MQ=255)
                                                                                       tGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                        <  2:257609/145‑1 (MQ=255)
                                                                                       tGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                        >  1:257609/1‑145 (MQ=255)
                                                                                       tGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCAGCGAAGAGGCCGGCACACACACAACAAAACCTAAGTTTTTATccagatct                                                    >  8:116378/1‑143 (MQ=255)
                                                                                         aacgACCGC‑‑AAGAAAGCTGCAGTAGCCGAACGCAA‑CACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACGGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                    <  2:344558/144‑1 (MQ=255)
                                                                                              cccc‑‑AAGTACGCTCCAGTAGCCCACCGCAC‑GAAGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTCCGCGGCCTGGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCa                                             <  5:415226/146‑1 (MQ=255)
                                                                                              ccGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCa                                             <  6:358758/149‑1 (MQ=255)
                                                                                                          gCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAAGAGGCCGGCCCAGACCCCCCAAAACCACAGTTTTTGTACGCCCGCGCCCGCAGCCCGTTAAg                                   >  6:275809/1‑149 (MQ=255)
                                                                                                           cgccAGTAGCCGAACGCAA‑GACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                  <  6:233001/146‑1 (MQ=255)
                                                                                                              ccgTAGCCGACCCCAA‑CAAGGCTGCCCGCGGCCAG‑CTTTTTTATTTTGTCCTGTGCCGCCCGCTGCGCGGCCTGGCCCCCGACGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                               <  4:407382/147‑1 (MQ=255)
                                                                                                              cAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                               <  6:79001/149‑1 (MQ=255)
                                                                                                                gaaGCCGAACGAAT‑TATGGCTGCCCTGGGGTAG‑CGTTTTTTATTTGTCCTGTGCCGGCGTCTTCGCGGCCTGGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCTCGCAGCCCTTTCAGGGTAtt                             <  1:124028/147‑1 (MQ=255)
                                                                                                                        tcGCAA‑GTAGGCTGCCCGCGGGCAG‑CTTTTTTTTTTTGTCTTGTGGCGGCTGTTTCGCGGCCTTGCGCGGTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                     <  5:4048/148‑1 (MQ=255)
                                                                                                                                  ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca            >  3:133438/1‑149 (MQ=255)
                                                                                                                                     tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg          >  7:359611/1‑148 (MQ=255)
                                                                                                                                             gggacgcTTTTTTTTTTTGTCCTGGCCCGCCCTCTGCCCGGCCTGGCCCGCTATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt  <  7:275148/143‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGC‑‑AAGTAAGCTGCAGTAGCCGAACGCAA‑GAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740660‑4740946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑