Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,499,508 +C intergenic (+85/‑79) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,499,5061.C96.2% 144.3 / 0.3 52intergenic (+83/‑81)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (23/27);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGG  >  NC_002947/1499360‑1499647
                                                                                                                                                       |                                                                                                                                             
cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑c                                                                                                                                              <  1:320270/149‑1 (MQ=37)
      aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑cccgctc                                                                                                                                        <  1:49109/149‑6 (MQ=25)
      aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑cccgctc                                                                                                                                        <  8:104194/149‑6 (MQ=25)
        cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTccc                                                                                                                                       <  2:95413/149‑1 (MQ=25)
        cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTccc                                                                                                                                       <  7:195750/149‑1 (MQ=25)
                gCGGCCATTCAGAGCCTCAACGAAGCCTTGGCACCACAGCAGGACTCAGGCGGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc                                                                                                                                 <  1:364308/147‑1 (MQ=18)
                  ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGAt                                                                                                                             >  7:310681/1‑149 (MQ=21)
                    ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTg                                                                                                                           <  7:219774/149‑1 (MQ=21)
                       tgcAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGAATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCa                                                                                                                        <  1:229803/147‑1 (MQ=21)
                        tCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGGAACAGGCGCCGCCGTGGATGGCACCGGCTTTGCCGGTGTT‑TGCGGGGA‑CGCCCGCGCCCACAGCCGGATTGGCaa                                                                                                                       >  7:241547/1‑148 (MQ=21)
                           gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTaa                                                                                                                    <  7:130286/149‑1 (MQ=21)
                                tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCCTGGCCCCGAAACCGGCGCCGCGGTGGATGGCACCGGCGTTGCCGGTGTT‑TGCCGGCACCC‑CCGCGCCCCCAGACTGCTTGGCATTAACCggg                                                                                                               >  3:38973/1‑149 (MQ=255)
                                 gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGGTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc                                                                                                                                 <  2:299681/130‑1 (MQ=18)
                                 gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc                                                                                                                                 >  1:299681/1‑130 (MQ=18)
                                    cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCA‑CGCCCCCGCCCACAGACTGATTGCCATTAAGCGGGCCt                                                                                                            >  2:71094/1‑148 (MQ=25)
                                      aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCGCCCCCAGACTGATTGCCATTAAGCGGGCCTATc                                                                                                         >  3:84272/1‑149 (MQ=21)
                                           ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                                                     >  3:70264/1‑148 (MQ=21)
                                               caccacAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                                                                <  8:310681/149‑1 (MQ=21)
                                                accacAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCCTGCCCCCCAAACAGGCCCCCCCGTGCATGGCACCCGCGTTTCCCGTGTTAG‑CCGGCACCC‑CCCCTCCCACAGACAGAAAGCCAATAAACGGGCCAATCGCAACAATg                                                                                                >  2:43450/1‑148 (MQ=255)
                                                  cacaGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                                                             >  5:19467/1‑149 (MQ=25)
                                                     agcagGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACCGCCGGCTTGGCATTAAGCGGGCCGATCGCTACAGTGACGGcc                                                                                          >  6:200489/1‑149 (MQ=18)
                                                                ggCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                               <  1:323950/149‑1 (MQ=21)
                                                                    accAAACGGGGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc                                                                                                         <  6:397926/118‑1 (MQ=17)
                                                                      cAAACGGGGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                                                        <  5:173726/118‑1 (MQ=17)
                                                                      cAAAAGGTGGGTTGCGGGCGTGTCCGCTAAACAGGCGCCGGGGTGCTTGGCACCGGGTTGGCCGGTGTTCG‑GGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                                           <  6:19467/148‑1 (MQ=21)
                                                                                  aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑TGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt                                                                       <  7:104956/140‑1 (MQ=21)
                                                                                  aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑TGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt                                                                       >  8:104956/1‑140 (MQ=21)
                                                                                     gggcgggcCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                           <  7:385436/149‑1 (MQ=37)
                                                                                     gggCGTGCCCGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                           <  4:38973/149‑1 (MQ=25)
                                                                                     gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCC‑CGCCCGCTCCCCCAGACTGAGTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGACTCAt                                                             >  1:377504/1‑147 (MQ=21)
                                                                                     gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAtt                                                            >  8:353712/1‑147 (MQ=25)
                                                                                       gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                                                        >  3:180129/1‑102 (MQ=17)
                                                                                       gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt                                                         >  8:140771/1‑149 (MQ=25)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                                                        >  3:278592/1‑128 (MQ=25)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                                                        <  4:278592/128‑1 (MQ=25)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                     >  4:56672/1‑147 (MQ=37)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                   <  1:43450/149‑1 (MQ=37)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                   <  8:241547/149‑1 (MQ=37)
                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                   <  4:70264/149‑1 (MQ=37)
                                                                                               gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg                                                  >  7:305335/1‑148 (MQ=37)
                                                                                                     cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                           <  7:140771/149‑1 (MQ=37)
                                                                                                      aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                           >  6:139808/1‑148 (MQ=37)
                                                                                                       ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                          >  8:306869/1‑148 (MQ=37)
                                                                                                          gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                      >  5:291918/1‑149 (MQ=37)
                                                                                                              cgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa                                  <  5:139808/149‑1 (MQ=37)
                                                                                                               ggTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt                                 <  1:71094/149‑1 (MQ=38)
                                                                                                                gTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                               >  5:36710/1‑102 (MQ=21)
                                                                                                                gTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                               <  6:36710/102‑1 (MQ=21)
                                                                                                                    aTGGCACCGGCTTTGCCGGTGTTCG‑CGGCCA‑CGCCCGCTCCCACACACTGATTGCCATTAAGCGGGCCTAACGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCAAACGAATCATCACGCCCTTACAACGCTCTACATCAGATCTGa                             >  8:289112/1‑148 (MQ=21)
                                                                                                                                  gCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct              >  5:166698/1‑149 (MQ=255)
                                                                                                                                       gtgttcgcGGGAA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATTTGAAACGTCCCGACTTCTTCCa          <  7:65502/142‑1 (MQ=255)
                                                                                                                                          ttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa          >  7:155458/5‑146 (MQ=255)
                                                                                                                                          ttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa          <  8:155458/142‑1 (MQ=255)
                                                                                                                                               gggCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg  <  7:306869/149‑1 (MQ=255)
                                                                                                                                               gggCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg  <  7:378962/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                             
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGG  >  NC_002947/1499360‑1499647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑