Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,932,6381.C98.1% 177.4 / ‑3.3 53intergenic (‑105/+45)rluA/minEbifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (26/26);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT  >  NC_002947/1932493‑1932781
                                                                                                                                                  |                                                                                                                                                
gcggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCCCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑ccc                                                                                                                                               <  1:163934/149‑2 (MQ=255)
         tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCTGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAggg                                                                                                                                       <  8:314060/148‑1 (MQ=255)
              gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGACATCAACTAAAAAGGATATTCGTACCCAGTAGGAGCGGGCTTGCCC‑CCGAATGGCTGCaa                                                                                                                                 >  3:231552/1‑149 (MQ=255)
                          actCGACAGCGGCATTGCAGGGCTCACCAAAATAGCAGTAACCGCTGGGGCCGCTTGGCGGCCCTGCGCGGGCACGCCCGCGCCTACAACGGATATGCGTCCCCGGTAGCCGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCaaa                                                                                                                     <  5:381611/147‑1 (MQ=255)
                          aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCaaa                                                                                                                     >  2:277438/1‑149 (MQ=255)
                               aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc                                                                                                                <  1:29237/149‑1 (MQ=255)
                                cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCg                                                                                                               <  2:42019/149‑1 (MQ=255)
                                     gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc                                                                                                                >  1:58067/1‑143 (MQ=255)
                                     gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc                                                                                                                <  2:58067/143‑1 (MQ=255)
                                     gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCACTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCAACAAAGGATATGCGTACCCGGTACGAGCGGGCTTGCCC‑GCGAACGGCTGCAAGGCAGCCCCAAATTGtagccaac                                                                                                          >  6:236301/1‑142 (MQ=255)
                                           cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa                                                                                                     >  2:346392/1‑148 (MQ=255)
                                           cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt                                                                                                    <  1:346392/149‑1 (MQ=255)
                                             ggTCTCACTAAAATAGCATTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGTATATGCGTCCCCTGTAGGTGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa                                                                                                  <  1:186531/149‑1 (MQ=37)
                                                   aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                            >  4:362153/1‑149 (MQ=255)
                                                   aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                            >  6:348227/1‑149 (MQ=255)
                                                   aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                            >  3:343167/1‑149 (MQ=255)
                                                       aaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGc                                                                                        <  5:444179/149‑1 (MQ=255)
                                                             cAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg                                                                                  <  4:113780/149‑1 (MQ=255)
                                                              aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa                                                                                 >  5:81766/1‑149 (MQ=255)
                                                              aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCCCGACGAAAAAGGATATGCGTCCCCAGTAGGCGCCGGCGTGCCC‑GCGAAGGGCGGCAAGGCAGCCCCAAAATTTGGACAAACCGGCTAAGCGATGCGGCAGGGTGa                                                                                 >  4:186955/1‑149 (MQ=255)
                                                               gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt                                                                                <  2:28395/149‑1 (MQ=255)
                                                                tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                               <  5:348227/149‑1 (MQ=255)
                                                                       tGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt                                                                        >  8:333423/1‑149 (MQ=255)
                                                                          ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                                     <  5:132354/149‑1 (MQ=255)
                                                                                tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc                                                                                                        >  7:41394/1‑108 (MQ=255)
                                                                                    cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca                                                           <  2:7571/149‑1 (MQ=255)
                                                                                    cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCGTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag                                                             >  3:338216/1‑147 (MQ=255)
                                                                                     agCCCTTCGCGGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                                >  8:404312/2‑143 (MQ=18)
                                                                                     agCCCTTCGCGGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                                <  7:404312/142‑1 (MQ=18)
                                                                                         cTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCCGCTCCAGAATCGAGCAGCTGc                                                      >  8:131015/1‑149 (MQ=255)
                                                                                             gcgGGCAAGCCCGCTCATACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg                                                  <  3:186955/149‑1 (MQ=255)
                                                                                                   aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                            <  6:81766/149‑1 (MQ=255)
                                                                                                   aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                            <  3:362153/149‑1 (MQ=255)
                                                                                                     gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCC‑GGTTTTCCAg                                         >  3:136439/1‑149 (MQ=255)
                                                                                                         gctgctAAAAAGTTTATGCTTCCCTTTTTGGTGGGGTCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc                                      <  4:231552/149‑1 (MQ=255)
                                                                                                         gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc                                                    <  7:55983/135‑1 (MQ=255)
                                                                                                         gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc                                                    >  8:55983/1‑135 (MQ=255)
                                                                                                             cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAAACGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                  >  4:79866/1‑149 (MQ=255)
                                                                                                               aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                                >  5:58177/1‑149 (MQ=255)
                                                                                                               aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                                >  7:87050/1‑149 (MQ=255)
                                                                                                                cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCAGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                                >  1:293248/1‑148 (MQ=255)
                                                                                                                aaaaGGATATGCGTCCCCTTTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                                <  5:329918/147‑1 (MQ=255)
                                                                                                                  aaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac                             <  7:333423/149‑1 (MQ=255)
                                                                                                                        atGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc                       >  3:200784/1‑149 (MQ=255)
                                                                                                                         ggCGTCCCCTGTCGGTGGGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc                       <  5:236301/147‑1 (MQ=255)
                                                                                                                              ccccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc                 >  3:374661/1‑149 (MQ=255)
                                                                                                                               cccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCC‑GGTTTTCCAGCTCGATATGCACATCATCGTTGcc                 <  4:136439/147‑1 (MQ=255)
                                                                                                                                    tAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc                                                                       <  2:414938/89‑1 (MQ=255)
                                                                                                                                    tAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc                                                                       >  1:414938/1‑89 (MQ=255)
                                                                                                                                    tAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt           >  8:406067/1‑149 (MQ=255)
                                                                                                                                    tAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt            <  4:200784/148‑1 (MQ=255)
                                                                                                                                             gCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCg                                     >  7:331145/1‑114 (MQ=255)
                                                                                                                                             gCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCg                                     <  8:331145/114‑1 (MQ=255)
                                                                                                                                             gCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt  >  7:334570/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT  >  NC_002947/1932493‑1932781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑