| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,057 | +C | intergenic (+140/+75) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,056 | 1 | . | C | 83.9% | 84.8 / 12.5 | 31 | intergenic (+139/+76) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base C (14/12); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑C‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947/4585923‑4586198 | gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTTGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCtt < 8:182651/148‑1 (MQ=38) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑t > 6:146117/1‑149 (MQ=39) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTgg < 7:132655/149‑1 (MQ=39) aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGa < 2:301549/147‑1 (MQ=39) tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCATGGCGGGTAAAACCCCAACAAACA‑GGGACC‑CTGCC‑C‑GCCAAGGA‑AC‑CGGCGCAGCCCCAGGGAGGCGCggg > 6:239176/1‑149 (MQ=255) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg < 7:157958/148‑1 (MQ=38) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg > 8:157958/1‑148 (MQ=38) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGG‑CAGCGCC‑‑‑GCATTCAA‑GGCCGGCACGGTCCCTGGTAGGAGCGGGt < 4:434254/148‑1 (MQ=14) ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAACTG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 1:52811/148‑1 (MQ=37) cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcgcg < 5:146117/149‑1 (MQ=38) ttGAAAGGTTGAGGATGACCGGTTGCTTGGCAAGGTGAAGATCTTGCATGGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGaa < 8:302248/149‑1 (MQ=37) aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCGGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga < 5:305045/148‑1 (MQ=37) gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg > 6:362630/1‑124 (MQ=37) gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg < 5:362630/124‑1 (MQ=37) gAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAAACCCCGCCGACCACGGGC‑CGGGGCCTGTGCCGTGAA‑TG‑CGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGCGGCCGGCCGCGCCAGCACATCAACCCg > 1:350018/1‑145 (MQ=16) aTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGc > 2:429073/1‑70 (MQ=17) aTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGc < 1:429073/70‑1 (MQ=17) ggTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGc > 1:195794/1‑149 (MQ=255) gTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCg > 2:367792/1‑149 (MQ=255) gTAAACCCGCTCCCACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAc > 2:401779/1‑103 (MQ=255) gTAAACCCGCTCCCACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAc < 1:401779/103‑1 (MQ=255) ccGCTCCTACCA‑GGGG‑CAGCGCC‑‑‑GCATTCAA‑GGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGc < 3:361547/122‑1 (MQ=14) ccGCTCCTACCA‑GGGG‑CAGCGCC‑‑‑GCATTCAA‑GGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGc > 4:361547/1‑122 (MQ=14) ccGCTCCTACCA‑GGGG‑CAGCGCC‑‑‑GCATTCAA‑GGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt > 2:3351/1‑148 (MQ=18) tCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTg > 1:298018/1‑148 (MQ=255) cTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAc > 3:59945/1‑149 (MQ=255) cTACCA‑GGGACC‑GTGCC‑G‑GCCTTCAA‑TG‑CGGCGCTCCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGCCCGGCCGAGCCAGCACATCACCACGAAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCAAGCGGAACTTCTGCAc > 8:333432/1‑149 (MQ=255) aCCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt > 4:169560/1‑149 (MQ=255) ccA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCtt > 4:317363/1‑149 (MQ=255) ccA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCt < 2:195794/148‑1 (MQ=255) cA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTg > 4:182672/1‑149 (MQ=255) | GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑C‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947/4585923‑4586198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |