| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,335,597 | +G | coding (2027/2976 nt) | PP_4695 → | two‑component system sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,335,596 | 1 | . | G | 98.3% | 204.2 / ‑3.1 | 59 | coding (2026/2976 nt) | PP_4695 | two‑component system sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (29/29); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GATGCCTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGC‑GCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATCGACGA > NC_002947/5335452‑5335735 | gATGCCTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTcgc‑ggcc > 3:197501/1‑146 (MQ=255) tGCCTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTcgc‑ggcctg < 8:368420/149‑6 (MQ=255) tGCCTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTcgc‑ggcctg < 5:435068/149‑6 (MQ=255) cTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTc < 3:349399/149‑1 (MQ=255) ttGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCa > 1:249643/1‑149 (MQ=255) tACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATcgacg > 6:46907/1‑149 (MQ=255) ccATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATcgacga < 2:249643/148‑1 (MQ=255) ccATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATcgacga < 3:433864/148‑1 (MQ=255) aCCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGcgcg < 1:431904/149‑1 (MQ=255) tGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCct > 5:35267/1‑149 (MQ=255) cAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCct < 8:101587/147‑1 (MQ=255) aGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCctgct > 3:37939/1‑149 (MQ=255) cATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTtc < 5:46907/149‑1 (MQ=255) aTGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTtca < 4:37939/149‑1 (MQ=255) tGGGCGTCTGCTCGCTGGCCAAGGACCAGTAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCTGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTtcat < 5:338181/149‑1 (MQ=255) tGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTtcat < 4:383137/149‑1 (MQ=255) gCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCaa > 2:166101/1‑149 (MQ=255) cGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAAc < 6:35267/149‑1 (MQ=255) cTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTg < 6:171136/146‑1 (MQ=255) cTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGc < 4:197501/147‑1 (MQ=255) cTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGc < 5:79942/147‑1 (MQ=255) cGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcga > 5:281661/1‑149 (MQ=255) tGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcgac > 3:252193/1‑147 (MQ=255) gCCAAGGACCAGGAACTCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcgacga < 6:47247/147‑1 (MQ=255) ccAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATcgacga > 1:325127/1‑94 (MQ=255) ccAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATcgacga < 2:325127/94‑1 (MQ=255) ggAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTgg > 6:45612/1‑78 (MQ=255) ggAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTgg < 5:45612/78‑1 (MQ=255) aTCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAagcagc < 6:281661/149‑1 (MQ=255) cTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGcc > 2:247422/1‑149 (MQ=255) aTGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTgg < 3:338956/149‑1 (MQ=255) gtgGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGgcg > 6:208666/1‑149 (MQ=255) gAACAAGGCCATGGAAGCGCTCACCGGCATCGCCGCCAAGCATGAGGTCGGCTCCCGGCCAGGTCACCATCGACGAGCCTTGGCGCGTCCTGCTGCAATGCTTCATCAACGTGCCCGCCGAGCACCTGCACAAGCAGCGCCTggaccg > 7:124336/1‑144 (MQ=255) gAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTg > 5:386815/1‑149 (MQ=255) gAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCGCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCt < 2:432009/148‑1 (MQ=255) ggCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcg < 8:372717/111‑1 (MQ=255) ggCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGc > 5:241890/1‑149 (MQ=255) ggCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGc > 4:352304/1‑149 (MQ=255) gCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGc > 1:78018/1‑148 (MQ=255) ccATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAagcagc > 5:352133/1‑130 (MQ=255) ccATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAagcagc < 6:352133/130‑1 (MQ=255) ggAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGcc < 1:387693/148‑1 (MQ=255) ggAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGcc > 2:387693/1‑148 (MQ=255) agagCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGC‑GCCCGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGCACGGCCAGCCGCgct > 3:296536/1‑149 (MQ=255) gCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcgacga < 5:141129/103‑1 (MQ=255) gCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCcgacga > 6:141129/1‑103 (MQ=255) cACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCgctggct > 3:1875/1‑148 (MQ=255) aCCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAgg > 5:327100/1‑78 (MQ=255) aCCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAgg < 6:327100/78‑1 (MQ=255) aCCGGCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCgctggctg > 5:148531/1‑148 (MQ=255) gCATCGCCGCCAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCt > 4:382819/1‑149 (MQ=255) gccgccAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGcac > 2:141917/1‑148 (MQ=255) ccgccAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGcac > 5:335050/1‑147 (MQ=255) ccAAGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAgg > 5:99396/1‑148 (MQ=255) aaGCATGTGGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAg < 6:99396/149‑1 (MQ=255) gtgGTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATc > 5:197982/1‑148 (MQ=255) ggTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATcgac < 4:252193/149‑1 (MQ=255) gTCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATcgac > 3:389726/1‑148 (MQ=255) tCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATcgacga < 3:382819/149‑1 (MQ=255) tCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATcgacga < 2:78018/149‑1 (MQ=255) tCGGCTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATcgacga < 5:208666/149‑1 (MQ=255) cTCGCGGCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACg > 8:143221/1‑102 (MQ=255) | GATGCCTTGCGCCGCTACCATCGCCAGACCCTGCAGGAGTTGCCCATGGGCGTCTGCTCGCTGGCCAAGGACCAGGAAATCCTGATGTGGAACAAGGCCATGGAAGAGCTCACCGGCATCGCCGCCAAGCATGTGGTCGGCTCGC‑GCCTGGTCACCATCGACGAGCCTTGGCGCGGCCTGCTGCAAGGCTTCATCAACGTGCCCGACGAGCACCTGCACAAGCAGCGCCTGGCGCTGGACGGCCAGCCGCGCTGGCTGAACCTGCACAAGGCAGCCATCGACGA > NC_002947/5335452‑5335735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |