Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947441,3260G.27.0% 88.7 / 33.3 37coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (17/10);  new base . (4/6);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCTGGTGG  >  NC_002947/441179‑441474
                                                                                                                                                      |                                                                                                                                                    
gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGAGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTgg                                                                                                                                                     <  5:202000/149‑1 (MQ=255)
           tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTggggggggggcct                                                                                                                                          >  5:154394/1‑146 (MQ=255)
            ggAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTggggggggggggcc                                                                                                                                         >  7:101935/1‑146 (MQ=255)
            ggAAAGCCCGCAAGGGCCGGAAGTGGCGGTCGACGGCCATAGGTTGATGGCCGTCTGAAGCAATGACTACCTGGGCCTTGCCAACCACCCCGAGGGGATAGCCGACTGG‑CAGGCCGGCG‑GCTCGCGCGTGGGT‑GTGGGGGGGGGGGCCt                                                                                                                                         >  4:112167/1‑149 (MQ=255)
                   ccccacgggCCGGAAGTGGGGGCCCCCGGCCCGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCGGCCCAACCCCCCCGCGGGGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTg                                                                                                                                 <  6:321760/143‑1 (MQ=255)
                   ccGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCt                                                                                                                                  >  5:321337/1‑149 (MQ=255)
                    cccAAGGGCCGGAAGGGGGGGGCCACGGCCCGCGGTTGCGGGCCTTGCTCATGAATGTCTACGGGGGCCGGGCCAACCACCCCCCTGTTTTCGGCGCCGGG‑CAGGCCGGCC‑CCGTGGGGGGGGGG‑GTGGGGGGGGGGGCCTCGCACCTg                                                                                                                                 <  2:83916/147‑1 (MQ=255)
                                aaGTGGTGGGCGACGGCCAGCGGTTGCGGGCCTTGTTCCGCAATGACTACCTGGGCGTGGCCAACCCCCCCGCGGGGTTCGCCGCCTGG‑CAGGCCGGCG‑CCGAGGGCGGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCcaca                                                                                                                    <  4:67079/149‑1 (MQ=255)
                                        gtCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCGGGTAGTTGGCCACGGcccccc                                                                                                             >  3:197757/1‑149 (MQ=255)
                                           gACGGCCTGCGGTTGCGGGCCTTCTGCAGAAATGCCTACGTGGGCCTGGCCAACCACCCCGTGGTGATCGCCGCCTGG‑CAGGGCGGGG‑CCGAGCGGTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc                                                                                                         <  6:13521/149‑1 (MQ=255)
                                              ggCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTgg                                                                                                                                >  7:288904/1‑124 (MQ=255)
                                                      gTTGCTGGCCTTGTGGACCATTGACTTCCGGGGCCGGGCCAACCACCCGGTGGTGATCGCGGCCTTG‑CATGGCGGGG‑GCGCGGGGTGGGGG‑GTGGGGGGGGAGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTg                                                                                               <  1:316606/149‑1 (MQ=255)
                                                       gtGCTGGCCTTCTGCCTCAATGACTAACTGGGCCTGGCCAACCACCCCGTGGTGCTCGCCCCCTGG‑CAGGCCGGCG‑CCGTGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGaa                                                                                             <  4:322306/148‑1 (MQ=255)
                                                         gggggCCTTGTGGAGCAATCCATACCGGGGCCTGGCCAACCCCCCCCTGGTTTTGGCCGCCGGGCCTGGCGGGCG‑‑CGTGGGGTGGGGG‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGaa                                                                                             <  2:26644/145‑1 (MQ=255)
                                                               cTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCAc                                                                                      >  3:180425/1‑149 (MQ=255)
                                                                  cTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACGTGGTAGTTGGCCAAAGCACCCCGCACAATCAGTTTGAAGGGGCAATg                                                                                    >  4:349981/1‑148 (MQ=255)
                                                                    gcagcaATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCGTCGCACCTGGTAGTTGGCCACCGCCCCCCGCACCAGCAGGTTGAAGCGGCACTGGCt                                                                                 >  8:309051/1‑149 (MQ=255)
                                                                            gcatACCGGGGCCTGGCCAACCACCCCGCGGTTATCGCCGCCTGG‑CAGGCCGGCG‑CCGTGCGGTGGGGT‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCAc                                                                         <  5:364591/146‑1 (MQ=255)
                                                                            gACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGTTTGAAGAGGCACTGGCTGAACTCAc                                                                         >  2:48278/1‑149 (MQ=255)
                                                                               tACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCgg                                                                      >  5:28381/1‑149 (MQ=255)
                                                                                 ccTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGCGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgcc                                                                    <  2:9142/149‑1 (MQ=255)
                                                                                 ccTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTAACCGgccg                                                                   >  7:266441/1‑149 (MQ=255)
                                                                                  cTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccg                                                                   >  3:144641/1‑149 (MQ=255)
                                                                                            ccAACCCCCCCGTGGTGTTCCCCCCCCGG‑CCGGGCGGCGCCCGGGCG‑GGGGGG‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGcc                                                         <  3:349981/149‑1 (MQ=255)
                                                                                              aaCCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCACCATCAGGTTGAAGAGGCACTGGGTGAACTCACCGGCCGCCCGCGCGCCCt                                                       >  1:56318/1‑149 (MQ=255)
                                                                                               accaccCCGTGGTGATCGCCGCCGGG‑CCGGCCGGCG‑CCGTGGGGTGGGGG‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgt                                                     <  4:220019/149‑1 (MQ=255)
                                                                                                  cccccctggTGATGGCCGCCGGG‑CTGGGCGGCG‑GCCTGGGGTGGGGTGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt                                                    <  8:101935/142‑1 (MQ=255)
                                                                                                   ccccGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCGCGCCCCTGGTAGTTGGCCACAGCACCCCCCACAAAACGGTTCAAGAGCCACTGGCTGCACTACCCGGCCGCCCGCGCGCCCggttgt                                                  >  6:235024/1‑149 (MQ=255)
                                                                                                   ccccGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTg                                                                                >  3:433357/1‑118 (MQ=255)
                                                                                                    cccGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgtt                                                 >  3:150612/1‑149 (MQ=255)
                                                                                                         ggggaccgccccCCGG‑CAGGCCGGCG‑CCCCGCCGGGGGGT‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCa                                            <  6:321337/143‑1 (MQ=255)
                                                                                                             aTCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑‑GGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGc                                       >  4:276395/1‑148 (MQ=255)
                                                                                                              tCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCGGGCACCTGGTAGTTGGCCACCGCCCCCCGCCCCATCAGGTGGAAGCGGCCAGGGGTGCCGTCCCCGGCCGCCCCGCCGCCCTGTTGTTGTCCACCggg                                       >  7:177461/1‑148 (MQ=255)
                                                                                                              gggccggcggg‑ccggcggGCG‑CCGCGCGCTGGGGT‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGc                                       <  7:309051/147‑1 (MQ=255)
                                                                                                                cgctcttGG‑CCGGCCGGCG‑CCGCGCGGGGGGGT‑GGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCta                                     <  6:192481/143‑1 (MQ=255)
                                                                                                                   gccTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATg                                 <  6:154394/149‑1 (MQ=255)
                                                                                                                      tGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCACCAACAGGTTGAAGAGCCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCGGGTGTTCACCCCCGGCTATATgg                                >  8:64250/1‑148 (MQ=255)
                                                                                                                       gg‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACGTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCtatacatcg                              >  5:192481/1‑144 (MQ=255)
                                                                                                                       gg‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GT‑GGGGGGGGGGCCTCGCACCTGGTATTTGGCCAAAGCACCCCGCACCATCAGGTTGAATAGGCACTGGATGCACACCCCGGCCGCCCGCCCGACCTGTTGTTCACCACCGGCAATATGGcc                              >  7:2499/1‑148 (MQ=255)
                                                                                                                                         cggggggggg‑gggggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCa              <  4:150612/144‑1 (MQ=255)
                                                                                                                                                 gt‑gtGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAACAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCCCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCtg      >  4:15733/1‑149 (MQ=255)
                                                                                                                                                      gggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCtggtgg  <  5:442516/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGG‑CAGGCCGGCG‑CCGAGCGCTGGGGT‑GTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCTGGTGG  >  NC_002947/441179‑441474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑