Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	220.7 / NA	64	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (33/31);  total (33/31)

CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA  >  NC_002947/5674612‑5674892                                                                                                                                                |                                                                                                                                            ccAGCGCTGCTCACCCGCAAGGGCTGATAGAAGTACCGCGGGGGCTGCTTCGCAGCCCTATCACGACACCAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                       <  3:263231/149‑1 (MQ=37)     cgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGttt                                                                                                                                   <  2:242096/149‑1 (MQ=255)           tgACCCGCAAGGTCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTATACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                             >  6:387415/1‑149 (MQ=37)            gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGttt                                                                                                                                   <  4:321251/142‑1 (MQ=255)            gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGttt                                                                                                                                   >  3:321251/1‑142 (MQ=255)             aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                           <  6:152598/149‑1 (MQ=255)              accgcgtgggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                           >  5:37381/8‑148 (MQ=37)               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                           >  7:225958/1‑147 (MQ=255)                         tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                               >  3:257424/1‑149 (MQ=255)                               aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCAGTGATAGGGCCGCAAAGCGGCCCCCGCGGtttt                                                                                                                                  <  1:59217/125‑1 (MQ=25)                               aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGtttt                                                                                                                                  >  2:59217/1‑124 (MQ=255)                               aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                         <  3:245377/149‑1 (MQ=255)                                  tACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCCGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac                                                                                                      <  3:277917/149‑1 (MQ=255)                                  tACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCCGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac                                                                                                      <  3:272264/149‑1 (MQ=255)                                    ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCacac                                                                                                    >  6:398497/1‑149 (MQ=255)                                           ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                              >  6:120059/1‑148 (MQ=255)                                               gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                         >  3:419857/1‑149 (MQ=255)                                                   cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTTCAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                                       <  7:283522/147‑1 (MQ=255)                                                   cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                                      <  6:88124/148‑1 (MQ=255)                                                     cAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc                                                                                   <  5:398497/149‑1 (MQ=255)                                                       gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                                 >  7:87057/1‑149 (MQ=255)                                                          cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTc                                                                                                                                 >  2:33017/1‑98 (MQ=255)                                                          cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTc                                                                                                                                 <  1:33017/98‑1 (MQ=255)                                                             tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                           <  4:419857/149‑1 (MQ=255)                                                              cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                                 >  1:138209/1‑110 (MQ=255)                                                              cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                                 <  2:138209/110‑1 (MQ=255)                                                              cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                          >  2:250799/1‑149 (MQ=255)                                                                  acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                      <  2:235068/149‑1 (MQ=255)                                                                    acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                           <  2:389506/110‑1 (MQ=255)                                                                    acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                           >  1:389506/1‑110 (MQ=255)                                                                      aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                   >  3:27088/1‑148 (MQ=255)                                                                       aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                                 <  5:120059/149‑1 (MQ=255)                                                                          ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                              <  2:14167/149‑1 (MQ=255)                                                                            gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                             >  1:60351/1‑148 (MQ=255)                                                                                 tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                       >  5:377349/1‑149 (MQ=255)                                                                                          tttCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                                <  8:315960/115‑1 (MQ=255)                                                                                          tttCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                                >  7:315960/1‑115 (MQ=255)                                                                                             cTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc                                           >  5:37437/1‑149 (MQ=255)                                                                                             cTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc                                           >  8:187567/1‑149 (MQ=255)                                                                                              ttGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                          >  6:339883/1‑149 (MQ=255)                                                                                              ttGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                          >  5:103730/1‑149 (MQ=255)                                                                                               tGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                          >  6:401384/1‑148 (MQ=255)                                                                                                gATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc                                           <  7:187567/146‑1 (MQ=255)                                                                                                 ttctcTGCAGGAGCGGCCTTGTGTC‑GTGATAGGGCTGCGAAGCAGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                       <  5:83318/148‑1 (MQ=37)                                                                                                      tACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                                <  7:395200/87‑1 (MQ=37)                                                                                                      tACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                                >  8:395200/1‑87 (MQ=37)                                                                                                      tACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                       <  6:103730/144‑1 (MQ=255)                                                                                                      tACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                       <  1:250799/144‑1 (MQ=255)                                                                                                       aCAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACGCTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                                 >  6:197588/1‑149 (MQ=255)                                                                                                       aCAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                                 >  7:209346/1‑149 (MQ=255)                                                                                                        cAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                                 >  7:110561/1‑148 (MQ=255)                                                                                                            aGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                            <  6:377349/149‑1 (MQ=255)                                                                                                              cGGCCTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                           <  4:27088/148‑1 (MQ=255)                                                                                                                 ccTTGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                       >  7:93253/1‑149 (MQ=255)                                                                                                                   ttGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                     >  8:103929/1‑149 (MQ=255)                                                                                                                   ttGTGTC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTAAGCgt                     >  3:285067/1‑149 (MQ=255)                                                                                                                      tgtC‑GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg                   <  8:87057/148‑1 (MQ=255)                                                                                                                                aGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt         >  4:409113/1‑149 (MQ=255)                                                                                                                                aGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt         >  3:112229/1‑149 (MQ=255)                                                                                                                                   gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTTCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa      <  5:387415/149‑1 (MQ=255)                                                                                                                                      gCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGcc   <  5:339883/149‑1 (MQ=255)                                                                                                                                      gCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc    >  1:17068/1‑148 (MQ=255)                                                                                                                                       cAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTGGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCTAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCa  <  6:37437/149‑1 (MQ=255)                                                                                                                                       cAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCa  <  4:257424/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                            CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTC‑GTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA  >  NC_002947/5674612‑5674892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑