Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	353,067	A→G	intergenic (+34/+84)	hisF → / ← cbcV	imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	353,067	0	A	G	100.0%	211.2 / NA	63	intergenic (+34/+84)	hisF/cbcV	imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/34);  total (29/34)

GGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑AGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACCA  >  NC_002947/352929‑353212                                                                                                                                            |                                                                                                                                                    gggCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCg                                                                                                                                           >  7:41596/1‑149 (MQ=255)   gCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCt                                                                                                                                         >  8:386794/1‑149 (MQ=255)    cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGcgc                                                                                                                                        >  3:83244/1‑147 (MQ=255)    cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAATGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGCTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTc                                                                                                                                        >  1:108848/1‑149 (MQ=255)       gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGGCCCGCCCGCGccc                                                                                                                                      >  1:199024/1‑149 (MQ=255)       gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCACCca                                                                                                                                     >  7:219051/1‑149 (MQ=255)       gCCAGCGCGGTGCTCGCGGCCAGCATCATCCACTTTGGCCAGTACACAGTCCCGGAGGCCAAGGCCTACATGGCTTCGCGCGCGATCGTCGTTCGCTGATCCAATGACCGGGCCTGCTATGCAGCCCTTCGC‑GGGCACGCCCGCTCCca                                                                                                                                     <  3:390847/149‑1 (MQ=38)        ccAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCcac                                                                                                                                    <  5:24499/149‑1 (MQ=255)         cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCca                                                                                                                                     >  5:214013/1‑147 (MQ=255)          aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAg                                                                                                                                  >  8:122684/1‑149 (MQ=255)          aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAg                                                                                                                                  <  3:106906/149‑1 (MQ=255)            cgcgGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGt                                                                                                                                >  7:34660/1‑149 (MQ=255)               ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTg                                                                                                                            <  3:271509/149‑1 (MQ=255)                 tGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCt                                                                                                                          <  1:245390/149‑1 (MQ=255)                   cTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCCGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTga                                                                                                                        <  8:88947/149‑1 (MQ=255)                    tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTgag                                                                                                                       <  5:189076/149‑1 (MQ=255)                         gcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGAtctc                                                                                                                  >  6:179931/1‑149 (MQ=255)                          ccAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATctct                                                                                                                 <  3:31209/149‑1 (MQ=255)                             gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCtgtg                                                                                                              <  8:34660/149‑1 (MQ=255)                              cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATctct                                                                                                                 >  3:155622/1‑145 (MQ=255)                              cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATctct                                                                                                                 <  4:155622/145‑1 (MQ=255)                              cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTgg                                                                                                             <  7:15416/149‑1 (MQ=255)                                  ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGGCCCCCCCCGCCCCCACGG‑GTTCTGTGATATCTGTGGGGGc                                                                                                         >  3:227072/1‑149 (MQ=255)                                  ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGc                                                                                                         >  7:334512/1‑149 (MQ=255)                                    ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCgg                                                                                                       >  7:253840/1‑149 (MQ=255)                                     cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCggg                                                                                                      <  2:165361/149‑1 (MQ=255)                                      aCTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGc                                                                                                     >  2:310342/1‑149 (MQ=255)                                       cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑ggg                                                                                                                                                   >  8:48994/1‑103 (MQ=255)                                       cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑ggg                                                                                                                                                   <  7:48994/103‑1 (MQ=255)                                            gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGt                                                                                                   >  7:5670/1‑145 (MQ=255)                                            gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGt                                                                                                   <  8:5670/145‑1 (MQ=255)                                            gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑ccc                                                                                              >  1:382718/1‑149 (MQ=255)                                            gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑ccc                                                                                              <  2:250363/149‑1 (MQ=255)                                             cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCcg                                                                                             <  2:382718/149‑1 (MQ=255)                                               aGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGTATTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCcgc                                                                                            <  1:59567/149‑1 (MQ=255)                                                 tACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCCCGCCCCCAGGG‑TTGCGGAGGTATAGGTGGGTGCGGGGGTG‑CCCGCGac                                                                                         >  8:379425/1‑148 (MQ=37)                                                      aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGcc                                                                                    >  2:241813/1‑149 (MQ=255)                                                      aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGcc                                                                                    <  6:214013/149‑1 (MQ=255)                                                      aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGAGCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGcc                                                                                    <  7:386794/149‑1 (MQ=255)                                                        tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCcgc                                                                                  <  5:179931/149‑1 (MQ=255)                                                        tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTGCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCcgc                                                                                  <  8:41596/149‑1 (MQ=255)                                                           cGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑ccc                                                                                              >  1:259239/1‑134 (MQ=255)                                                           cGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑ccc                                                                                              <  2:259239/134‑1 (MQ=255)                                                               ggCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCgg                                                                           <  8:334512/149‑1 (MQ=255)                                                                        cTACATGGGTTCGGGCGGGAGCGTCGTTCGCTGATCCATTGCCCGGGGCTGGTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGACATCTCTGTGGGAGGGGGGGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTg                                                                  <  4:83244/149‑1 (MQ=38)                                                                           cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGc                                                                                                                           >  3:219145/1‑90 (MQ=255)                                                                           cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGc                                                                                                                           <  4:219145/90‑1 (MQ=255)                                                                           cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGc                                                                 <  1:335737/147‑1 (MQ=255)                                                                            aTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                               >  1:44200/1‑148 (MQ=255)                                                                            aTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCGGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTc                                                              <  2:108848/149‑1 (MQ=255)                                                                                        gggATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTc                                                   <  1:310342/149‑1 (MQ=255)                                                                                                  ttCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCt                                                                >  4:73571/1‑125 (MQ=255)                                                                                                  ttCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCt                                                                <  3:73571/125‑1 (MQ=255)                                                                                                  ttCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcag                                        <  8:253840/149‑1 (MQ=255)                                                                                                      cTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCCCTCCCACAGGT‑TTGCTGAGATATCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCATCAAACGTGGTTCTTTCCGAGCAGcgcg                                    >  4:342153/1‑149 (MQ=38)                                                                                                       tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCCCAGGT‑TTGCTGAGATGTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGt                                   >  4:49995/1‑149 (MQ=255)                                                                                                       tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGt                                   >  8:214793/1‑149 (MQ=255)                                                                                                         aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCCCAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGcgcggt                                  >  5:186547/1‑146 (MQ=255)                                                                                                         aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTgg                                 >  1:277674/1‑149 (MQ=255)                                                                                                         aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTgg                                 <  7:122684/149‑1 (MQ=255)                                                                                                            cATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                                     <  7:359358/142‑1 (MQ=255)                                                                                                             aTTGACCGGGGCTGCTTTGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTa                               >  1:49902/1‑147 (MQ=255)                                                                                                                  ccGGGGCTGCTTGGCAGCCGTGCGC‑GGGCACGCCCGCTCCCACAGGT‑TTCCTTAGATCTCTGGGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                        <  7:379425/149‑1 (MQ=38)                                                                                                                              tGCAGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa              <  3:152499/147‑1 (MQ=255)                                                                                                                                 aGCCCTTCGC‑GGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATc         <  2:277674/149‑1 (MQ=255)                                                                                                                                        cgc‑gGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCccacca  >  3:334093/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                    GGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGC‑AGGCACGCCCGCTCCCACAGGT‑TTGCTGAGATCTCTGTGGGAGCGGGCGTG‑CCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACCA  >  NC_002947/352929‑353212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑