| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 93.8% | 124.4 / ‑3.9 | 50 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); major base C (19/26); minor base A (3/0); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.0% | 134.4 / ‑5.8 | 50 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (22/26); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA > NC_002947/1499360‑1499639 ||| cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑c < 1:320270/149‑1 (MQ=37) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑cccgctc < 1:49109/149‑6 (MQ=25) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑cg‑cccgctc < 8:104194/149‑6 (MQ=25) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTccc < 2:95413/149‑1 (MQ=25) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTccc < 7:195750/149‑1 (MQ=25) gCGGCCATTCAGAGCCTCAACGAAGCCTTGGCACCACAGCAGGACTCAGGCGGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc < 1:364308/147‑1 (MQ=18) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGAt > 7:310681/1‑149 (MQ=21) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTg < 7:219774/149‑1 (MQ=21) tgcAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGAATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCa < 1:229803/147‑1 (MQ=21) tCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGGAACAGGCGCCGCCGTGGATGGCACCGGCTTTGCCGGTGTT‑TGCGGGGA‑CGCCCGCGCCCACAGCCGGATTGGCaa > 7:241547/1‑148 (MQ=21) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTaa < 7:130286/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCCTGGCCCCGAAACCGGCGCCGCGGTGGATGGCACCGGCGTTGCCGGTGTT‑TGCCGGCACCC‑CCGCGCCCCCAGACTGCTTGGCATTAACCggg > 3:38973/1‑149 (MQ=255) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGGTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc < 2:299681/130‑1 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGAc > 1:299681/1‑130 (MQ=18) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCA‑CGCCCCCGCCCACAGACTGATTGCCATTAAGCGGGCCt > 2:71094/1‑148 (MQ=25) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCGCCCCCAGACTGATTGCCATTAAGCGGGCCTATc > 3:84272/1‑149 (MQ=21) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 3:70264/1‑148 (MQ=21) caccacAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg < 8:310681/149‑1 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCCTGCCCCCCAAACAGGCCCCCCCGTGCATGGCACCCGCGTTTCCCGTGTTAG‑CCGGCACCC‑CCCCTCCCACAGACAGAAAGCCAATAAACGGGCCAATCGCAACAATg > 2:43450/1‑148 (MQ=255) cacaGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg > 5:19467/1‑149 (MQ=25) agcagGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACCGCCGGCTTGGCATTAAGCGGGCCGATCGCTACAGTGACGGcc > 6:200489/1‑149 (MQ=18) ggCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 1:323950/149‑1 (MQ=21) accAAACGGGGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 6:397926/118‑1 (MQ=17) cAAACGGGGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 5:173726/118‑1 (MQ=17) cAAAAGGTGGGTTGCGGGCGTGTCCGCTAAACAGGCGCCGGGGTGCTTGGCACCGGGTTGGCCGGTGTTCG‑GGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt < 6:19467/148‑1 (MQ=21) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑TGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt < 7:104956/140‑1 (MQ=21) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑TGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt > 8:104956/1‑140 (MQ=21) gggcgggcCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 7:385436/149‑1 (MQ=37) gggCGTGCCCGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 4:38973/149‑1 (MQ=25) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCC‑CGCCCGCTCCCCCAGACTGAGTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGACTCAt > 1:377504/1‑147 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAtt > 8:353712/1‑147 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg > 3:180129/1‑102 (MQ=17) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 8:140771/1‑149 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 3:278592/1‑128 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 4:278592/128‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:56672/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:43450/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 8:241547/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 4:70264/149‑1 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 7:305335/1‑148 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 7:140771/149‑1 (MQ=37) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 6:139808/1‑148 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 8:306869/1‑148 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:291918/1‑149 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa < 5:139808/149‑1 (MQ=37) ggTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt < 1:71094/149‑1 (MQ=38) gTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 5:36710/1‑102 (MQ=21) gTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 6:36710/102‑1 (MQ=21) aTGGCACCGGCTTTGCCGGTGTTCG‑CGGCCA‑CGCCCGCTCCCACACACTGATTGCCATTAAGCGGGCCTAACGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCAAACGAATCATCACGCCCTTACAACGCTCTACATCAGATCTGa > 8:289112/1‑148 (MQ=21) gCCGGTGTTCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct > 5:166698/1‑149 (MQ=255) gtgttcgcGGGAA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATTTGAAACGTCCCGACTTCTTCCa < 7:65502/142‑1 (MQ=255) ttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 7:155458/5‑146 (MQ=255) ttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 8:155458/142‑1 (MQ=255) ||| CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGG‑AGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA > NC_002947/1499360‑1499639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |